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10.1515/cclm-2020-0548 http://scihub22266oqcxt.onion/10.1515/cclm-2020-0548 32609640!ä!32609640 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Chem+Lab+Med 2020 ; 58 (9): 1601-1607 Nephropedia Template TP {{tp|p=32609640|t=2020. Development and multicenter performance evaluation of fully automated SARS-CoV-2 IgM and IgG immunoassays |pdf=|usr=}}{{32609640}} gab.com Text Development and multicenter performance evaluation of fully automated SARS-CoV-2 IgM and IgG immunoassays JO: Clin Chem Lab Med http://www.kidney.de/pget.php?&tw=1&pmid=32609640 #FOAvip OBJECTIVES: The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread globally. The laboratory diagnosis of SARS-CoV-2 infection has relied on nucleic acid testing; however, it has some limitations, such as low throughput and high rates of false negatives. Tests of higher sensitivity are needed to effectively identify infected patients. METHODS: This study has developed fully automated chemiluminescent immunoassays to determine IgM and IgG antibodies to SARS-CoV-2 in human serum. The assay performance has been evaluated at 10 hospitals. Clinical specificity was evaluated by measuring 972 hospitalized patients and 586 donors of a normal population. Clinical sensitivity was assessed on 513 confirmed cases of SARS-CoV-2 by RT-PCR. RESULTS: The assays demonstrated satisfied assay precision with coefficient of variation of less than 4.45%. Inactivation of specimen did not affect assay measurement. SARS-CoV-2 IgM showed clinical specificity of 97.33 and 99.49% for hospitalized patients and the normal population respectively, and SARS-CoV-2 IgG showed clinical specificity of 97.43 and 99.15% respectively. SARS-CoV-2 IgM showed clinical sensitivity of 82.54, 92.93, and 84.62% before 7 days, 7-14 days, and after 14 days respectively, since onset of symptoms, and SARS-CoV-2 IgG showed clinical sensitivity of 80.95, 97.98, and 99.15% respectively at the same time points above. CONCLUSIONS: We have developed fully automated immunoassays for detecting SARS-CoV-2 IgM and IgG antibodies in human serum. The assays demonstrated high clinical specificity and sensitivity, and add great value to nucleic acid testing in fighting against the global pandemic of the SARS-CoV-2 infection. Twit Text FOAVip Development and multicenter performance evaluation of fully automated SARS-CoV-2 IgM and IgG immunoassays ????Clin Chem Lab Med http://www.kidney.de/pget.php?&tw=1&pmid=32609640 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32609640 #FOAvip English Wikipedia <ref>{{Cite pmid|32609640}}</ref> Development and multicenter performance evaluation of fully automated SARS-CoV-2 IgM and IgG immunoassays #MMPMID32609640 Qian C; Zhou M; Cheng F; Lin X; Gong Y; Xie X; Li P; Li Z; Zhang P; Liu Z; Hu F; Wang Y; Li Q; Zhu Y; Duan G; Xing Y; Song H; Xu W; Liu BF; Xia F Clin Chem Lab Med 2020[Aug]; 58 (9): 1601-1607 PMID32609640show ga OBJECTIVES: The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread globally. The laboratory diagnosis of SARS-CoV-2 infection has relied on nucleic acid testing; however, it has some limitations, such as low throughput and high rates of false negatives. Tests of higher sensitivity are needed to effectively identify infected patients. METHODS: This study has developed fully automated chemiluminescent immunoassays to determine IgM and IgG antibodies to SARS-CoV-2 in human serum. The assay performance has been evaluated at 10 hospitals. Clinical specificity was evaluated by measuring 972 hospitalized patients and 586 donors of a normal population. Clinical sensitivity was assessed on 513 confirmed cases of SARS-CoV-2 by RT-PCR. RESULTS: The assays demonstrated satisfied assay precision with coefficient of variation of less than 4.45%. Inactivation of specimen did not affect assay measurement. SARS-CoV-2 IgM showed clinical specificity of 97.33 and 99.49% for hospitalized patients and the normal population respectively, and SARS-CoV-2 IgG showed clinical specificity of 97.43 and 99.15% respectively. SARS-CoV-2 IgM showed clinical sensitivity of 82.54, 92.93, and 84.62% before 7 days, 7-14 days, and after 14 days respectively, since onset of symptoms, and SARS-CoV-2 IgG showed clinical sensitivity of 80.95, 97.98, and 99.15% respectively at the same time points above. CONCLUSIONS: We have developed fully automated immunoassays for detecting SARS-CoV-2 IgM and IgG antibodies in human serum. The assays demonstrated high clinical specificity and sensitivity, and add great value to nucleic acid testing in fighting against the global pandemic of the SARS-CoV-2 infection. |Adolescent[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |Antibodies, Viral/blood/immunology[MESH] |Betacoronavirus/*immunology[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Child[MESH] |Child, Preschool[MESH] |Clinical Laboratory Techniques[MESH] |Coronavirus Infections/*diagnosis[MESH] |Humans[MESH] |Immunoassay/*methods[MESH] |Immunoglobulin G/*blood/immunology[MESH] |Immunoglobulin M/*blood/immunology[MESH] |Infant[MESH] |Middle Aged[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH]Development and multicenter performance evaluation of fully automated (epmc).pdf DeepDyve Pubget Overpricing