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10.1590/S1678-9946202062044

http://scihub22266oqcxt.onion/10.1590/S1678-9946202062044
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32609256!7325591!32609256
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suck abstract from ncbi


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pmid32609256      Rev+Inst+Med+Trop+Sao+Paulo 2020 ; 62 (ä): e44
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  • SARS-CoV-2 and the COVID-19 disease: a mini review on diagnostic methods #MMPMID32609256
  • Oliveira BA; Oliveira LC; Sabino EC; Okay TS
  • Rev Inst Med Trop Sao Paulo 2020[]; 62 (ä): e44 PMID32609256show ga
  • Coronavirus disease 2019 (COVID-19) is an infectious disease initially reported in China and currently worldwide dispersed caused by a new coronavirus (SARS-CoV-2 or 2019-nCoV) affecting more than seven million people around the world causing more than 400 thousand deaths (on June 8th, 2020). The diagnosis of COVID-19 is based on the clinical and epidemiological history of the patient. However, the gold standard for COVID-19 diagnosis is the viral detection through the amplification of nucleic acids. Although the quantitative Reverse-Transcription Polymerase Chain Reaction (RT-PCR) has been described as the gold standard for diagnosing COVID-19, there are several difficulties involving its use. Here we comment on RT-PCR and describe alternative tests developed for the diagnosis of COVID-19.
  • |*Pandemics[MESH]
  • |Betacoronavirus/genetics/*isolation & purification[MESH]
  • |COVID-19[MESH]
  • |Coronavirus Infections/*diagnosis/*epidemiology[MESH]
  • |Enzyme-Linked Immunosorbent Assay/methods[MESH]
  • |Humans[MESH]
  • |Immunoassay/methods[MESH]
  • |Immunoenzyme Techniques/methods[MESH]
  • |Nucleic Acid Amplification Techniques/methods/standards[MESH]
  • |Pneumonia, Viral/*diagnosis/*epidemiology[MESH]
  • |Protein Array Analysis/methods[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction/methods[MESH]
  • |SARS-CoV-2[MESH]


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