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10.1016/j.ijid.2020.06.074

http://scihub22266oqcxt.onion/10.1016/j.ijid.2020.06.074
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32599282!7318955!32599282
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suck abstract from ncbi


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pmid32599282      Int+J+Infect+Dis 2020 ; 98 (ä): 16-17
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  • Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification #MMPMID32599282
  • Fukumoto T; Iwasaki S; Fujisawa S; Hayasaka K; Sato K; Oguri S; Taki K; Nakakubo S; Kamada K; Yamashita Y; Konno S; Nishida M; Sugita J; Teshima T
  • Int J Infect Dis 2020[Sep]; 98 (ä): 16-17 PMID32599282show ga
  • Rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for the diagnosis of coronavirus disease 2019 (COVID-19) and preventing the spread of the virus. A novel detection kit - the 2019 Novel Coronavirus Detection Kit (nCoV-DK) - halves the detection time by eliminating the steps of RNA extraction and purification. We evaluated the concordance between the nCoV-DK and direct PCR. The virus was detected in 53/71 specimens (74.6%) by direct PCR and in 55/71 specimens (77.5%) by nCoV-DK; the overall concordance rate was 94.4%: 95.2% for nasopharyngeal swab, 95.5% for saliva, and 85.7% for sputum. The nCoV-DK test effectively detects SARS-CoV-2 in all types of sample including saliva, while reducing the time required for detection, labor, and the risk of human error.
  • |Betacoronavirus/*genetics[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Clinical Laboratory Techniques/*methods[MESH]
  • |Coronavirus Infections/*diagnosis/*virology[MESH]
  • |Diagnostic Tests, Routine/*methods[MESH]
  • |Humans[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis/*virology[MESH]
  • |Polymerase Chain Reaction[MESH]
  • |RNA, Viral/isolation & purification[MESH]
  • |SARS-CoV-2[MESH]
  • |Saliva/virology[MESH]


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