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10.1002/jcla.23411 http://scihub22266oqcxt.onion/10.1002/jcla.23411 32594577!7361150!32594577     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Lab+Anal 2020 ; 34 (9): e23411 Nephropedia Template TP {{tp|p=32594577|t=2020. Heat inactivation of serum interferes with the immunoanalysis of antibodies to SARS-CoV-2 |pdf=|usr=}}{{32594577}} gab.com Text Heat inactivation of serum interferes with the immunoanalysis of antibodies to SARS-CoV-2 JO: J Clin Lab Anal http://www.kidney.de/pget.php?&tw=1&pmid=32594577 #FOAvip BACKGROUND: The detection of serum antibodies to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is emerging as a new tool for the coronavirus disease 2019 (COVID-19) diagnosis. Since many coronaviruses are sensitive to heat, heating inactivation of samples at 56 degrees C prior to testing is considered a possible method to reduce the risk of transmission, but the effect of heating on the measurement of SARS-CoV-2 antibodies is still unclear. METHODS: By comparing the levels of SARS-CoV-2 antibodies before and after heat inactivation of serum at 56 degrees C for 30 minutes using a quantitative fluorescence immunochromatographic assay RESULTS: We showed that heat inactivation significantly interferes with the levels of antibodies to SARS-CoV-2. The IgM levels of all the 34 serum samples (100%) from COVID-19 patients decreased by an average level of 53.56%. The IgG levels were decreased in 22 of 34 samples (64.71%) by an average level of 49.54%. Similar changes can also be observed in the non-COVID-19 disease group (n = 9). Of note, 44.12% of the detected IgM levels were dropped below the cutoff value after heating, suggesting heat inactivation can lead to false-negative results of these samples. CONCLUSION: Our results indicate that heat inactivation of serum at 56 degrees C for 30 minutes interferes with the immunoanalysis of antibodies to SARS-CoV-2. Heat inactivation prior to immunoanalysis is not recommended, and the possibility of false-negative results should be considered if the sample was pre-inactivated by heating. Twit Text FOAVip Heat inactivation of serum interferes with the immunoanalysis of antibodies to SARS-CoV-2 ????J Clin Lab Anal http://www.kidney.de/pget.php?&tw=1&pmid=32594577 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32594577 #FOAvip English Wikipedia <ref>{{Cite pmid|32594577}}</ref> Heat inactivation of serum interferes with the immunoanalysis of antibodies to SARS-CoV-2 #MMPMID32594577 Hu X; An T; Situ B; Hu Y; Ou Z; Li Q; He X; Zhang Y; Tian P; Sun D; Rui Y; Wang Q; Ding D; Zheng L J Clin Lab Anal 2020[Sep]; 34 (9): e23411 PMID32594577show ga BACKGROUND: The detection of serum antibodies to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is emerging as a new tool for the coronavirus disease 2019 (COVID-19) diagnosis. Since many coronaviruses are sensitive to heat, heating inactivation of samples at 56 degrees C prior to testing is considered a possible method to reduce the risk of transmission, but the effect of heating on the measurement of SARS-CoV-2 antibodies is still unclear. METHODS: By comparing the levels of SARS-CoV-2 antibodies before and after heat inactivation of serum at 56 degrees C for 30 minutes using a quantitative fluorescence immunochromatographic assay RESULTS: We showed that heat inactivation significantly interferes with the levels of antibodies to SARS-CoV-2. The IgM levels of all the 34 serum samples (100%) from COVID-19 patients decreased by an average level of 53.56%. The IgG levels were decreased in 22 of 34 samples (64.71%) by an average level of 49.54%. Similar changes can also be observed in the non-COVID-19 disease group (n = 9). Of note, 44.12% of the detected IgM levels were dropped below the cutoff value after heating, suggesting heat inactivation can lead to false-negative results of these samples. CONCLUSION: Our results indicate that heat inactivation of serum at 56 degrees C for 30 minutes interferes with the immunoanalysis of antibodies to SARS-CoV-2. Heat inactivation prior to immunoanalysis is not recommended, and the possibility of false-negative results should be considered if the sample was pre-inactivated by heating. |*Hot Temperature[MESH] |Antibodies, Viral/*immunology[MESH] |Betacoronavirus/*immunology[MESH] |COVID-19[MESH] |Coronavirus Infections/*blood/*immunology/virology[MESH] |Humans[MESH] |Immunoassay/*methods[MESH] |Immunoglobulin G/blood[MESH] |Immunoglobulin M/blood[MESH] |Pandemics[MESH] |Pneumonia, Viral/*blood/*immunology/virology[MESH] |SARS-CoV-2[MESH]Heat inactivation of serum interferes with the immunoanalysis of antib(epmc).pdf DeepDyve Pubget Overpricing