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10.1093/infdis/jiaa370

http://scihub22266oqcxt.onion/10.1093/infdis/jiaa370
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32594170!7337811!32594170
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suck abstract from ncbi


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pmid32594170      J+Infect+Dis 2020 ; 222 (6): 899-902
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  • Suboptimal Biological Sampling as a Probable Cause of False-Negative COVID-19 Diagnostic Test Results #MMPMID32594170
  • Kinloch NN; Ritchie G; Brumme CJ; Dong W; Dong W; Lawson T; Jones RB; Montaner JSG; Leung V; Romney MG; Stefanovic A; Matic N; Lowe CF; Brumme ZL
  • J Infect Dis 2020[Aug]; 222 (6): 899-902 PMID32594170show ga
  • False-negative severe acute respiratory syndrome coronavirus 2 test results can negatively impact the clinical and public health response to coronavirus disease 2019 (COVID-19). We used droplet digital polymerase chain reaction (ddPCR) to demonstrate that human DNA levels, a stable molecular marker of sampling quality, were significantly lower in samples from 40 confirmed or suspected COVID-19 cases that yielded negative diagnostic test results (ie, suspected false-negative test results) compared with a representative pool of 87 specimens submitted for COVID-19 testing. Our results support suboptimal biological sampling as a contributor to false-negative COVID-19 test results and underscore the importance of proper training and technique in the collection of nasopharyngeal specimens.
  • |Betacoronavirus/genetics/*isolation & purification[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Clinical Laboratory Techniques/*methods[MESH]
  • |Coronavirus Infections/*diagnosis[MESH]
  • |False Negative Reactions[MESH]
  • |Humans[MESH]
  • |Nasopharynx/virology[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis[MESH]
  • |RNA, Viral/genetics/isolation & purification[MESH]
  • |Real-Time Polymerase Chain Reaction/methods[MESH]
  • |SARS-CoV-2[MESH]
  • |Specimen Handling/*methods[MESH]


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