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Room-temperature-storable PCR mixes for SARS-CoV-2 detection #MMPMID32592724
Xu J; Wang J; Zhong Z; Su X; Yang K; Chen Z; Zhang D; Li T; Wang Y; Zhang S; Ge S; Zhang J; Xia N
Clin Biochem 2020[Oct]; 84 (ä): 73-78 PMID32592724show ga
OBJECTIVES: A novel coronavirus (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) emerged in late 2019, causing an outbreak of pneumonia [coronavirus disease 2019 (COVID-19)] globally. Although the use of ready-made reaction mixes can enable more rapid PCR-based diagnosis of COVID-19, the need to transport and store these mixes at low temperatures presents challenges to already overburdened logistics networks. METHODS: Here, we present an optimized freeze-drying procedure that allows SARS-CoV-2 PCR mixes to be transported and stored at ambient temperatures, without loss of activity. Additive-supplemented PCR mixes were freeze-dried. The residual moisture of the freeze-dried PCR mixes was measured by Karl-Fischer titration. RESULTS: We found that the freeze-dried PCR mixes with ~1.2% residual moisture are optimal for storage, transport, and reconstitution. The sensitivity, specificity, and repeatability of the freeze-dried reagents were similar to those of freshly prepared, wet reagents. The freeze-dried mixes retained activity at room temperature (18 ~ 25 degrees C) for 28 days, and for 14 and 10 days when stored at 37 degrees C and 56 degrees C, respectively. CONCLUSION: The uptake of this approach will ease logistical challenges faced by transport networks and make more cold storage space available at diagnosis and hospital laboratories.
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Clin+Biochem 2020 ; 84 (ä): 73-78
