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10.1016/j.cmi.2020.06.009 http://scihub22266oqcxt.onion/10.1016/j.cmi.2020.06.009 32585353!7308776!32585353     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Microbiol+Infect 2020 ; 26 (9): 1248-1253 Nephropedia Template TP {{tp|p=32585353|t=2020. Large-scale implementation of pooled RNA extraction and RT-PCR for SARS-CoV-2 detection |pdf=|usr=}}{{32585353}} gab.com Text Large-scale implementation of pooled RNA extraction and RT-PCR for SARS-CoV-2 detection JO: Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=32585353 #FOAvip INTRODUCTION: Testing for active SARS-CoV-2 infection is a fundamental tool in the public health measures taken to control the COVID-19 pandemic. Because of the overwhelming use of SARS-CoV-2 reverse transcription (RT)-PCR tests worldwide, the availability of test kits has become a major bottleneck and the need to increase testing throughput is rising. We aim to overcome these challenges by pooling samples together, and performing RNA extraction and RT-PCR in pools. METHODS: We tested the efficiency and sensitivity of pooling strategies for RNA extraction and RT-PCR detection of SARS-CoV-2. We tested 184 samples both individually and in pools to estimate the effects of pooling. We further implemented Dorfman pooling with a pool size of eight samples in large-scale clinical tests. RESULTS: We demonstrated pooling strategies that increase testing throughput while maintaining high sensitivity. A comparison of 184 samples tested individually and in pools of eight samples showed that test results were not significantly affected. Implementing the eight-sample Dorfman pooling to test 26 576 samples from asymptomatic individuals, we identified 31 (0.12%) SARS-CoV-2 positive samples, achieving a 7.3-fold increase in throughput. DISCUSSION: Pooling approaches for SARS-CoV-2 testing allow a drastic increase in throughput while maintaining clinical sensitivity. We report the successful large-scale pooled screening of asymptomatic populations. Twit Text FOAVip Large-scale implementation of pooled RNA extraction and RT-PCR for SARS-CoV-2 detection ????Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=32585353 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32585353 #FOAvip English Wikipedia <ref>{{Cite pmid|32585353}}</ref> Large-scale implementation of pooled RNA extraction and RT-PCR for SARS-CoV-2 detection #MMPMID32585353 Ben-Ami R; Klochendler A; Seidel M; Sido T; Gurel-Gurevich O; Yassour M; Meshorer E; Benedek G; Fogel I; Oiknine-Djian E; Gertler A; Rotstein Z; Lavi B; Dor Y; Wolf DG; Salton M; Drier Y Clin Microbiol Infect 2020[Sep]; 26 (9): 1248-1253 PMID32585353show ga INTRODUCTION: Testing for active SARS-CoV-2 infection is a fundamental tool in the public health measures taken to control the COVID-19 pandemic. Because of the overwhelming use of SARS-CoV-2 reverse transcription (RT)-PCR tests worldwide, the availability of test kits has become a major bottleneck and the need to increase testing throughput is rising. We aim to overcome these challenges by pooling samples together, and performing RNA extraction and RT-PCR in pools. METHODS: We tested the efficiency and sensitivity of pooling strategies for RNA extraction and RT-PCR detection of SARS-CoV-2. We tested 184 samples both individually and in pools to estimate the effects of pooling. We further implemented Dorfman pooling with a pool size of eight samples in large-scale clinical tests. RESULTS: We demonstrated pooling strategies that increase testing throughput while maintaining high sensitivity. A comparison of 184 samples tested individually and in pools of eight samples showed that test results were not significantly affected. Implementing the eight-sample Dorfman pooling to test 26 576 samples from asymptomatic individuals, we identified 31 (0.12%) SARS-CoV-2 positive samples, achieving a 7.3-fold increase in throughput. DISCUSSION: Pooling approaches for SARS-CoV-2 testing allow a drastic increase in throughput while maintaining clinical sensitivity. We report the successful large-scale pooled screening of asymptomatic populations. |Betacoronavirus/genetics/*isolation & purification[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/*methods[MESH] |Coronavirus Infections/*diagnosis/epidemiology[MESH] |Diagnostic Tests, Routine[MESH] |Humans[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/epidemiology[MESH] |RNA, Viral/genetics[MESH] |Reproducibility of Results[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH]Large-scale implementation of pooled RNA extraction and RT-PCR for SAR(epmc).pdf DeepDyve Pubget Overpricing