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Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Curr+Protoc+Microbiol 2020 ; 58 (1): e108 Nephropedia Template TP
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An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening #MMPMID32585083
Amanat F; White KM; Miorin L; Strohmeier S; McMahon M; Meade P; Liu WC; Albrecht RA; Simon V; Martinez-Sobrido L; Moran T; Garcia-Sastre A; Krammer F
Curr Protoc Microbiol 2020[Sep]; 58 (1): e108 PMID32585083show ga
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in the city of Wuhan, Hubei Province, China, in late 2019. Since then, the virus has spread globally and caused a pandemic. Assays that can measure the antiviral activity of antibodies or antiviral compounds are needed for SARS-CoV-2 vaccine and drug development. Here, we describe in detail a microneutralization assay, which can be used to assess in a quantitative manner if antibodies or drugs can block entry and/or replication of SARS-CoV-2 in vitro. (c) 2020 Wiley Periodicals LLC. Basic Protocol 1: Microneutralization assay to test inhibition of virus by antibodies (purified antibodies or serum/plasma) Basic Protocol 2: Screening of anti-SARS-CoV-2 compounds in vitro Support Protocol: SARS-CoV-2 propagation.