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10.1002/cpmc.108

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32585083!7361222!32585083
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suck abstract from ncbi


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pmid32585083      Curr+Protoc+Microbiol 2020 ; 58 (1): e108
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  • An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening #MMPMID32585083
  • Amanat F; White KM; Miorin L; Strohmeier S; McMahon M; Meade P; Liu WC; Albrecht RA; Simon V; Martinez-Sobrido L; Moran T; Garcia-Sastre A; Krammer F
  • Curr Protoc Microbiol 2020[Sep]; 58 (1): e108 PMID32585083show ga
  • The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in the city of Wuhan, Hubei Province, China, in late 2019. Since then, the virus has spread globally and caused a pandemic. Assays that can measure the antiviral activity of antibodies or antiviral compounds are needed for SARS-CoV-2 vaccine and drug development. Here, we describe in detail a microneutralization assay, which can be used to assess in a quantitative manner if antibodies or drugs can block entry and/or replication of SARS-CoV-2 in vitro. (c) 2020 Wiley Periodicals LLC. Basic Protocol 1: Microneutralization assay to test inhibition of virus by antibodies (purified antibodies or serum/plasma) Basic Protocol 2: Screening of anti-SARS-CoV-2 compounds in vitro Support Protocol: SARS-CoV-2 propagation.
  • |Animals[MESH]
  • |Antibodies, Viral/*immunology[MESH]
  • |Betacoronavirus/*immunology[MESH]
  • |COVID-19[MESH]
  • |Chlorocebus aethiops[MESH]
  • |Coronavirus Infections/immunology/virology[MESH]
  • |Drug Evaluation, Preclinical/*methods[MESH]
  • |Humans[MESH]
  • |Mice[MESH]
  • |Neutralization Tests/*methods[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/immunology/virology[MESH]
  • |SARS-CoV-2[MESH]
  • |Vero Cells[MESH]
  • |Virus Internalization/drug effects[MESH]


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