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10.1002/eji.202048715 http://scihub22266oqcxt.onion/10.1002/eji.202048715 32584420!7361618!32584420     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32584420&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Eur+J+Immunol 2020 ; 50 (8): 1234-1236 Nephropedia Template TP {{tp|p=32584420|t=2020. LIPS method for the detection of SARS-CoV-2 antibodies to spike and nucleocapsid proteins |pdf=|usr=}}{{32584420}} gab.com Text LIPS method for the detection of SARS-CoV-2 antibodies to spike and nucleocapsid proteins JO: Eur J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=32584420 #FOAvip Profiling antibodies to SARS-CoV-2 can help to assess potential immune response after COVID-19 disease. Luciferase IP system (LIPS) assay is a sensitive method for quantitative detection of antibodies to antigens in their native conformation. We here describe LIPS to detect antibody responses to SARS-CoV-2 spike (S) and nucleocapsid (N) proteins in COVID-19 patients. The antibodies targeted both S and N fragments and gave a high assay sensitivity by identifying 26 out of 26 COVID-19 patients with N antigen or with three protein fragments when combined into a single reaction. The assay correlated well with ELISA method and was specific to COVID-19 as we saw no reactivity among uninfected healthy controls. Our results show that LIPS is a rapid and measurable method to screen antibody responses against SARS-CoV-2 antigens. Twit Text FOAVip LIPS method for the detection of SARS-CoV-2 antibodies to spike and nucleocapsid proteins ????Eur J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=32584420 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32584420 #FOAvip English Wikipedia <ref>{{Cite pmid|32584420}}</ref> LIPS method for the detection of SARS-CoV-2 antibodies to spike and nucleocapsid proteins #MMPMID32584420 Haljasmagi L; Remm A; Rumm AP; Krassohhina E; Sein H; Tamm A; Kisand K; Peterson P Eur J Immunol 2020[Aug]; 50 (8): 1234-1236 PMID32584420show ga Profiling antibodies to SARS-CoV-2 can help to assess potential immune response after COVID-19 disease. Luciferase IP system (LIPS) assay is a sensitive method for quantitative detection of antibodies to antigens in their native conformation. We here describe LIPS to detect antibody responses to SARS-CoV-2 spike (S) and nucleocapsid (N) proteins in COVID-19 patients. The antibodies targeted both S and N fragments and gave a high assay sensitivity by identifying 26 out of 26 COVID-19 patients with N antigen or with three protein fragments when combined into a single reaction. The assay correlated well with ELISA method and was specific to COVID-19 as we saw no reactivity among uninfected healthy controls. Our results show that LIPS is a rapid and measurable method to screen antibody responses against SARS-CoV-2 antigens. |*Antibodies, Viral/blood/immunology[MESH] |*Betacoronavirus/immunology/metabolism[MESH] |*Coronavirus Infections/blood/immunology[MESH] |*Nucleocapsid Proteins/immunology/metabolism[MESH] |*Pandemics[MESH] |*Pneumonia, Viral/blood/immunology[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |COVID-19[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH]LIPS method for the detection of SARS-CoV-2 antibodies to spike and nu(epmc).pdf DeepDyve Pubget Overpricing