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Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Euro+Surveill 2020 ; 25 (24): ä Nephropedia Template TP
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Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020 #MMPMID32583765
Muenchhoff M; Mairhofer H; Nitschko H; Grzimek-Koschewa N; Hoffmann D; Berger A; Rabenau H; Widera M; Ackermann N; Konrad R; Zange S; Graf A; Krebs S; Blum H; Sing A; Liebl B; Wolfel R; Ciesek S; Drosten C; Protzer U; Boehm S; Keppler OT
Euro Surveill 2020[Jun]; 25 (24): ä PMID32583765show ga
Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven diagnostic laboratories in Germany in March 2020. While most assays performed well, we identified detection problems in a commonly used assay that may have resulted in false-negative test results during the first weeks of the pandemic.