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10.1002/smll.202002169

http://scihub22266oqcxt.onion/10.1002/smll.202002169
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32578378!7361204!32578378
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suck abstract from ncbi


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pmid32578378      Small 2020 ; 16 (32): e2002169
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  • Nanopore Targeted Sequencing for the Accurate and Comprehensive Detection of SARS-CoV-2 and Other Respiratory Viruses #MMPMID32578378
  • Wang M; Fu A; Hu B; Tong Y; Liu R; Liu Z; Gu J; Xiang B; Liu J; Jiang W; Shen G; Zhao W; Men D; Deng Z; Yu L; Wei W; Li Y; Liu T
  • Small 2020[Aug]; 16 (32): e2002169 PMID32578378show ga
  • The ongoing global novel coronavirus pneumonia COVID-19 outbreak has engendered numerous cases of infection and death. COVID-19 diagnosis relies upon nucleic acid detection; however, currently recommended methods exhibit high false-negative rates and are unable to identify other respiratory virus infections, thereby resulting in patient misdiagnosis and impeding epidemic containment. Combining the advantages of targeted amplification and long-read, real-time nanopore sequencing, herein, nanopore targeted sequencing (NTS) is developed to detect SARS-CoV-2 and other respiratory viruses simultaneously within 6-10 h, with a limit of detection of ten standard plasmid copies per reaction. Compared with its specificity for five common respiratory viruses, the specificity of NTS for SARS-CoV-2 reaches 100%. Parallel testing with approved real-time reverse transcription-polymerase chain reaction kits for SARS-CoV-2 and NTS using 61 nucleic acid samples from suspected COVID-19 cases show that NTS identifies more infected patients (22/61) as positive, while also effectively monitoring for mutated nucleic acid sequences, categorizing types of SARS-CoV-2, and detecting other respiratory viruses in the test sample. NTS is thus suitable for COVID-19 diagnosis; moreover, this platform can be further extended for diagnosing other viruses and pathogens.
  • |*Nanopores[MESH]
  • |Betacoronavirus/classification/*genetics/*isolation & purification[MESH]
  • |COVID-19[MESH]
  • |Coronavirus Infections/*diagnosis/epidemiology/*virology[MESH]
  • |DNA, Viral/genetics/isolation & purification[MESH]
  • |Genes, Viral[MESH]
  • |Humans[MESH]
  • |Limit of Detection[MESH]
  • |Mutation[MESH]
  • |Nanotechnology[MESH]
  • |Nucleic Acid Amplification Techniques/*methods/statistics & numerical data[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis/epidemiology/*virology[MESH]
  • |RNA, Viral/genetics/isolation & purification[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]
  • |Respiratory Tract Infections/diagnosis/virology[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction[MESH]
  • |SARS-CoV-2[MESH]


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