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10.3390/ijms21124396 http://scihub22266oqcxt.onion/10.3390/ijms21124396 32575728!7352576!32575728     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Mol+Sci 2020 ; 21 (12): ä Nephropedia Template TP {{tp|p=32575728|t=2020. Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellular SARS-CoV-2 RNAs |pdf=|usr=}}{{32575728}} gab.com Text Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellular SARS-CoV-2 RNAs JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=32575728 #FOAvip The novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. Meanwhile, increased demand for testing has led to a shortage of reagents and supplies and compromised the performance of diagnostic laboratories in many countries. Both the World Health Organization (WHO) and the Center for Disease Control and Prevention (CDC) recommend multi-step RT-PCR assays using multiple primer and probe pairs, which might complicate the interpretation of the test results, especially for borderline cases. In this study, we describe an alternative RT-PCR approach for the detection of SARS-CoV-2 RNA that can be used for the probe-based detection of clinical isolates in diagnostics as well as in research labs using a low-cost SYBR green method. For the evaluation, we used samples from patients with confirmed SARS-CoV-2 infections and performed RT-PCR assays along with successive dilutions of RNA standards to determine the limit of detection. We identified an M-gene binding primer and probe pair highly suitable for the quantitative detection of SARS-CoV-2 RNA for diagnostic and research purposes. Twit Text FOAVip Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellular SARS-CoV-2 RNAs ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=32575728 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32575728 #FOAvip English Wikipedia <ref>{{Cite pmid|32575728}}</ref> Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellular SARS-CoV-2 RNAs #MMPMID32575728 Toptan T; Hoehl S; Westhaus S; Bojkova D; Berger A; Rotter B; Hoffmeier K; Cinatl J Jr; Ciesek S; Widera M Int J Mol Sci 2020[Jun]; 21 (12): ä PMID32575728show ga The novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. Meanwhile, increased demand for testing has led to a shortage of reagents and supplies and compromised the performance of diagnostic laboratories in many countries. Both the World Health Organization (WHO) and the Center for Disease Control and Prevention (CDC) recommend multi-step RT-PCR assays using multiple primer and probe pairs, which might complicate the interpretation of the test results, especially for borderline cases. In this study, we describe an alternative RT-PCR approach for the detection of SARS-CoV-2 RNA that can be used for the probe-based detection of clinical isolates in diagnostics as well as in research labs using a low-cost SYBR green method. For the evaluation, we used samples from patients with confirmed SARS-CoV-2 infections and performed RT-PCR assays along with successive dilutions of RNA standards to determine the limit of detection. We identified an M-gene binding primer and probe pair highly suitable for the quantitative detection of SARS-CoV-2 RNA for diagnostic and research purposes. |Animals[MESH] |Betacoronavirus/genetics[MESH] |COVID-19 Testing[MESH] |Caco-2 Cells[MESH] |Chlorocebus aethiops[MESH] |Clinical Laboratory Techniques/economics/*methods/standards[MESH] |Coronavirus Infections/diagnosis/economics[MESH] |Coronavirus M Proteins[MESH] |Costs and Cost Analysis[MESH] |Humans[MESH] |RNA, Viral/chemistry/genetics[MESH] |Reverse Transcriptase Polymerase Chain Reaction/economics/*methods/standards[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH] |Vero Cells[MESH]Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellu(epmc).pdf DeepDyve Pubget Overpricing