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10.1515/cclm-2020-0749 http://scihub22266oqcxt.onion/10.1515/cclm-2020-0749 32573469!ä!32573469 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Chem+Lab+Med 2020 ; 58 (9): 1579-1586 Nephropedia Template TP {{tp|p=32573469|t=2020. SARS-CoV-2 RNA identification in nasopharyngeal swabs: issues in pre-analytics |pdf=|usr=}}{{32573469}} gab.com Text SARS-CoV-2 RNA identification in nasopharyngeal swabs: issues in pre-analytics JO: Clin Chem Lab Med http://www.kidney.de/pget.php?&tw=1&pmid=32573469 #FOAvip OBJECTIVES: The direct identification of SARS-CoV-2 RNA in nasopharyngeal swabs is recommended for diagnosing the novel COVID-19 disease. Pre-analytical determinants, such as sampling procedures, time and temperature storage conditions, might impact on the end result. Our aim was to evaluate the effects of sampling procedures, time and temperature of the primary nasopharyngeal swabs storage on real-time reverse-transcription polymerase chain reaction (rRT-PCR) results. METHODS: Each nasopharyngeal swab obtained from 10 hospitalized patients for COVID-19 was subdivided in 15 aliquots: five were kept at room temperature; five were refrigerated (+4 degrees C); five were immediately mixed with the extraction buffer and refrigerated at +4 degrees C. Every day and for 5 days, one aliquot per condition was analyzed (rRT-PCR) for SARS-CoV-2 gene E and RNaseP and threshold cycles (Ct) compared. To evaluate manual sampling, 70 nasopharyngeal swabs were sampled twice by two different operators and analyzed separately one from the other. RESULTS: A total of 6/10 swabs were SARS-CoV-2 positive. No significant time or storage-dependent variations were observed in SARS-CoV-2 Ct. Re-sampling of swabs with SARS-CoV-2 Ct lower than 33 resulted in highly reproducible results (CV=2.9%), while a high variability was observed when Ct values were higher than 33 (CV=10.3%). CONCLUSIONS: This study demonstrates that time and temperature of nasopharyngeal swabs storage do not significantly impact on results reproducibility. However, swabs sampling is a critical step, and especially in case of low viral load, might be a potential source of diagnostic errors. Twit Text FOAVip SARS-CoV-2 RNA identification in nasopharyngeal swabs: issues in pre-analytics ????Clin Chem Lab Med http://www.kidney.de/pget.php?&tw=1&pmid=32573469 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32573469 #FOAvip English Wikipedia <ref>{{Cite pmid|32573469}}</ref> SARS-CoV-2 RNA identification in nasopharyngeal swabs: issues in pre-analytics #MMPMID32573469 Basso D; Aita A; Navaglia F; Franchin E; Fioretto P; Moz S; Bozzato D; Zambon CF; Martin B; Dal Pra C; Crisanti A; Plebani M Clin Chem Lab Med 2020[Aug]; 58 (9): 1579-1586 PMID32573469show ga OBJECTIVES: The direct identification of SARS-CoV-2 RNA in nasopharyngeal swabs is recommended for diagnosing the novel COVID-19 disease. Pre-analytical determinants, such as sampling procedures, time and temperature storage conditions, might impact on the end result. Our aim was to evaluate the effects of sampling procedures, time and temperature of the primary nasopharyngeal swabs storage on real-time reverse-transcription polymerase chain reaction (rRT-PCR) results. METHODS: Each nasopharyngeal swab obtained from 10 hospitalized patients for COVID-19 was subdivided in 15 aliquots: five were kept at room temperature; five were refrigerated (+4 degrees C); five were immediately mixed with the extraction buffer and refrigerated at +4 degrees C. Every day and for 5 days, one aliquot per condition was analyzed (rRT-PCR) for SARS-CoV-2 gene E and RNaseP and threshold cycles (Ct) compared. To evaluate manual sampling, 70 nasopharyngeal swabs were sampled twice by two different operators and analyzed separately one from the other. RESULTS: A total of 6/10 swabs were SARS-CoV-2 positive. No significant time or storage-dependent variations were observed in SARS-CoV-2 Ct. Re-sampling of swabs with SARS-CoV-2 Ct lower than 33 resulted in highly reproducible results (CV=2.9%), while a high variability was observed when Ct values were higher than 33 (CV=10.3%). CONCLUSIONS: This study demonstrates that time and temperature of nasopharyngeal swabs storage do not significantly impact on results reproducibility. However, swabs sampling is a critical step, and especially in case of low viral load, might be a potential source of diagnostic errors. |Aged[MESH] |Aged, 80 and over[MESH] |Betacoronavirus/*chemistry[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques[MESH] |Coronavirus Envelope Proteins[MESH] |Coronavirus Infections/diagnosis[MESH] |Diagnostic Tests, Routine[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Nasopharynx/*virology[MESH] |Pandemics[MESH] |Pneumonia, Viral/diagnosis[MESH] |RNA, Viral/*analysis[MESH] |Reproducibility of Results[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |Ribonuclease P/genetics[MESH] |SARS-CoV-2[MESH] |Specimen Handling/*methods[MESH] |Temperature[MESH] |Time Factors[MESH]SARS-CoV-2 RNA identification in nasopharyngeal swabs: issues in pre-a(epmc).pdf DeepDyve Pubget Overpricing