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10.1021/acs.jproteome.0c00280

http://scihub22266oqcxt.onion/10.1021/acs.jproteome.0c00280
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32568543!ä!32568543

suck abstract from ncbi


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pmid32568543      J+Proteome+Res 2020 ; 19 (11): 4389-4392
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  • Mass Spectrometric Identification of SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients #MMPMID32568543
  • Ihling C; Tanzler D; Hagemann S; Kehlen A; Huttelmaier S; Arlt C; Sinz A
  • J Proteome Res 2020[Nov]; 19 (11): 4389-4392 PMID32568543show ga
  • Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. The protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. Data are available via ProteomeXchange with identifier PXD019423.
  • |Betacoronavirus/*chemistry[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Chromatography, High Pressure Liquid[MESH]
  • |Clinical Laboratory Techniques[MESH]
  • |Coronavirus Infections/*diagnosis/virology[MESH]
  • |Humans[MESH]
  • |Mass Spectrometry/*methods[MESH]
  • |Mouth/*virology[MESH]
  • |Nucleoproteins/analysis/chemistry[MESH]
  • |Pandemics[MESH]
  • |Peptide Fragments/analysis/chemistry[MESH]
  • |Pneumonia, Viral/*diagnosis/virology[MESH]
  • |SARS-CoV-2[MESH]


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