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10.1039/d0an00629g http://scihub22266oqcxt.onion/10.1039/d0an00629g 32568341!ä!32568341 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Analyst 2020 ; 145 (15): 5345-5352 Nephropedia Template TP {{tp|p=32568341|t=2020. Development of a lateral flow immunoassay strip for rapid detection of IgG antibody against SARS-CoV-2 virus |pdf=|usr=}}{{32568341}} gab.com Text Development of a lateral flow immunoassay strip for rapid detection of IgG antibody against SARS-CoV-2 virus JO: Analyst http://www.kidney.de/pget.php?&tw=1&pmid=32568341 #FOAvip The ongoing worldwide SARS-CoV-2 epidemic clearly has a tremendous influence on public health. Molecular detection based on oral swabs was used for confirmation of SARS-CoV-2 infection. However, high false negative rates were reported. We describe here the development of a point-of-care (POC) serological assay for the detection of IgG antibody against SARS-CoV-2. The principle of a lateral flow immunoassay strip (LFIAs) consists of fixing SARS-CoV-2 nucleocapsid protein to the surface of the strip and coupling anti-human IgG with colloidal gold nanoparticles (Au NPs). A series of parameters of this method were optimized, including the concentration of coating antigen, BSA blocking concentration and pH value for conjugation. The entire detection process took 15-20 min with a volume of 80 muL of the analyte solution containing 10 muL of serum and 70 muL sample diluent. The performance of the established assay was evaluated using serum samples of the clinically diagnosed cases of Coronavirus Disease 2019 (COVID-19). Our results indicated that the LFIAs for SARS-CoV-2 had satisfactory stability and reproducibility. As a result, our fast and easy LFIAs could provide a preliminary test result for physicians to make the correct diagnosis of SARS-CoV-2 infections along with alternative testing methods and clinical findings, as well as seroprevalence determination, especially in low-resource countries. Twit Text FOAVip Development of a lateral flow immunoassay strip for rapid detection of IgG antibody against SARS-CoV-2 virus ????Analyst http://www.kidney.de/pget.php?&tw=1&pmid=32568341 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32568341 #FOAvip English Wikipedia <ref>{{Cite pmid|32568341}}</ref> Development of a lateral flow immunoassay strip for rapid detection of IgG antibody against SARS-CoV-2 virus #MMPMID32568341 Wen T; Huang C; Shi FJ; Zeng XY; Lu T; Ding SN; Jiao YJ Analyst 2020[Aug]; 145 (15): 5345-5352 PMID32568341show ga The ongoing worldwide SARS-CoV-2 epidemic clearly has a tremendous influence on public health. Molecular detection based on oral swabs was used for confirmation of SARS-CoV-2 infection. However, high false negative rates were reported. We describe here the development of a point-of-care (POC) serological assay for the detection of IgG antibody against SARS-CoV-2. The principle of a lateral flow immunoassay strip (LFIAs) consists of fixing SARS-CoV-2 nucleocapsid protein to the surface of the strip and coupling anti-human IgG with colloidal gold nanoparticles (Au NPs). A series of parameters of this method were optimized, including the concentration of coating antigen, BSA blocking concentration and pH value for conjugation. The entire detection process took 15-20 min with a volume of 80 muL of the analyte solution containing 10 muL of serum and 70 muL sample diluent. The performance of the established assay was evaluated using serum samples of the clinically diagnosed cases of Coronavirus Disease 2019 (COVID-19). Our results indicated that the LFIAs for SARS-CoV-2 had satisfactory stability and reproducibility. As a result, our fast and easy LFIAs could provide a preliminary test result for physicians to make the correct diagnosis of SARS-CoV-2 infections along with alternative testing methods and clinical findings, as well as seroprevalence determination, especially in low-resource countries. |Antibodies, Viral/blood[MESH] |Betacoronavirus/*isolation & purification/metabolism[MESH] |COVID-19[MESH] |Coronavirus Infections/*diagnosis/virology[MESH] |Coronavirus Nucleocapsid Proteins[MESH] |Gold/chemistry[MESH] |Humans[MESH] |Immunoassay/*methods[MESH] |Immunoglobulin G/*blood[MESH] |Immunoglobulin M/blood[MESH] |Metal Nanoparticles/chemistry[MESH] |Nucleocapsid Proteins/immunology[MESH] |Pandemics[MESH] |Phosphoproteins[MESH] |Pneumonia, Viral/*diagnosis/virology[MESH] |Point-of-Care Systems[MESH] |Reproducibility of Results[MESH]Development of a lateral flow immunoassay strip for rapid detection of(epmc).pdf DeepDyve Pubget Overpricing