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10.1099/jgv.0.001453 http://scihub22266oqcxt.onion/10.1099/jgv.0.001453 32568027!7654748!32568027     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Gen+Virol 2020 ; 101 (9): 925-940 Nephropedia Template TP {{tp|p=32568027|t=2020. SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology |pdf=|usr=}}{{32568027}} gab.com Text SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology JO: J Gen Virol http://www.kidney.de/pget.php?&tw=1&pmid=32568027 #FOAvip The sudden emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at the end of 2019 from the Chinese province of Hubei and its subsequent pandemic spread highlight the importance of understanding the full molecular details of coronavirus infection and pathogenesis. Here, we compared a variety of replication features of SARS-CoV-2 and SARS-CoV and analysed the cytopathology caused by the two closely related viruses in the commonly used Vero E6 cell line. Compared to SARS-CoV, SARS-CoV-2 generated higher levels of intracellular viral RNA, but strikingly about 50-fold less infectious viral progeny was recovered from the culture medium. Immunofluorescence microscopy of SARS-CoV-2-infected cells established extensive cross-reactivity of antisera previously raised against a variety of non-structural proteins, membrane and nucleocapsid protein of SARS-CoV. Electron microscopy revealed that the ultrastructural changes induced by the two SARS viruses are very similar and occur within comparable time frames after infection. Furthermore, we determined that the sensitivity of the two viruses to three established inhibitors of coronavirus replication (remdesivir, alisporivir and chloroquine) is very similar, but that SARS-CoV-2 infection was substantially more sensitive to pre-treatment of cells with pegylated interferon alpha. An important difference between the two viruses is the fact that - upon passaging in Vero E6 cells - SARS-CoV-2 apparently is under strong selection pressure to acquire adaptive mutations in its spike protein gene. These mutations change or delete a putative furin-like cleavage site in the region connecting the S1 and S2 domains and result in a very prominent phenotypic change in plaque assays. Twit Text FOAVip SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology ????J Gen Virol http://www.kidney.de/pget.php?&tw=1&pmid=32568027 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32568027 #FOAvip English Wikipedia <ref>{{Cite pmid|32568027}}</ref> SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology #MMPMID32568027 Ogando NS; Dalebout TJ; Zevenhoven-Dobbe JC; Limpens RWAL; van der Meer Y; Caly L; Druce J; de Vries JJC; Kikkert M; Barcena M; Sidorov I; Snijder EJ J Gen Virol 2020[Sep]; 101 (9): 925-940 PMID32568027show ga The sudden emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at the end of 2019 from the Chinese province of Hubei and its subsequent pandemic spread highlight the importance of understanding the full molecular details of coronavirus infection and pathogenesis. Here, we compared a variety of replication features of SARS-CoV-2 and SARS-CoV and analysed the cytopathology caused by the two closely related viruses in the commonly used Vero E6 cell line. Compared to SARS-CoV, SARS-CoV-2 generated higher levels of intracellular viral RNA, but strikingly about 50-fold less infectious viral progeny was recovered from the culture medium. Immunofluorescence microscopy of SARS-CoV-2-infected cells established extensive cross-reactivity of antisera previously raised against a variety of non-structural proteins, membrane and nucleocapsid protein of SARS-CoV. Electron microscopy revealed that the ultrastructural changes induced by the two SARS viruses are very similar and occur within comparable time frames after infection. Furthermore, we determined that the sensitivity of the two viruses to three established inhibitors of coronavirus replication (remdesivir, alisporivir and chloroquine) is very similar, but that SARS-CoV-2 infection was substantially more sensitive to pre-treatment of cells with pegylated interferon alpha. An important difference between the two viruses is the fact that - upon passaging in Vero E6 cells - SARS-CoV-2 apparently is under strong selection pressure to acquire adaptive mutations in its spike protein gene. These mutations change or delete a putative furin-like cleavage site in the region connecting the S1 and S2 domains and result in a very prominent phenotypic change in plaque assays. |Adaptation, Biological[MESH] |Animals[MESH] |Antibodies, Viral/immunology[MESH] |Betacoronavirus/genetics/*physiology[MESH] |Cell Line/ultrastructure/virology[MESH] |Chlorocebus aethiops[MESH] |Computational Biology[MESH] |Conserved Sequence[MESH] |Cross Reactions[MESH] |Cytopathogenic Effect, Viral[MESH] |High-Throughput Nucleotide Sequencing[MESH] |Humans[MESH] |Immune Sera/immunology[MESH] |Kinetics[MESH] |Mice[MESH] |Microscopy, Electron[MESH] |Microscopy, Fluorescence[MESH] |RNA, Viral/isolation & purification[MESH] |Rabbits[MESH] |SARS-CoV-2[MESH] |Severe acute respiratory syndrome-related coronavirus/*physiology[MESH] |Vero Cells/ultrastructure/virology[MESH]SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics,(epmc).pdf DeepDyve Pubget Overpricing