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10.1016/j.tibtech.2020.05.012 http://scihub22266oqcxt.onion/10.1016/j.tibtech.2020.05.012 32564882!7302792!32564882     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Trends+Biotechnol 2020 ; 38 (10): 1066-1098 Nephropedia Template TP {{tp|p=32564882|t=2020. Technologies and Standardization in Research on Extracellular Vesicles |pdf=|usr=}}{{32564882}} gab.com Text Technologies and Standardization in Research on Extracellular Vesicles JO: Trends Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=32564882 #FOAvip Extracellular vesicles (EVs) are phospholipid bilayer membrane-enclosed structures containing RNAs, proteins, lipids, metabolites, and other molecules, secreted by various cells into physiological fluids. EV-mediated transfer of biomolecules is a critical component of a variety of physiological and pathological processes. Potential applications of EVs in novel diagnostic and therapeutic strategies have brought increasing attention. However, EV research remains highly challenging due to the inherently complex biogenesis of EVs and their vast heterogeneity in size, composition, and origin. There is a need for the establishment of standardized methods that address EV heterogeneity and sources of pre-analytical and analytical variability in EV studies. Here, we review technologies developed for EV isolation and characterization and discuss paths toward standardization in EV research. Twit Text FOAVip Technologies and Standardization in Research on Extracellular Vesicles ????Trends Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=32564882 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32564882 #FOAvip English Wikipedia <ref>{{Cite pmid|32564882}}</ref> Technologies and Standardization in Research on Extracellular Vesicles #MMPMID32564882 Gandham S; Su X; Wood J; Nocera AL; Alli SC; Milane L; Zimmerman A; Amiji M; Ivanov AR Trends Biotechnol 2020[Oct]; 38 (10): 1066-1098 PMID32564882show ga Extracellular vesicles (EVs) are phospholipid bilayer membrane-enclosed structures containing RNAs, proteins, lipids, metabolites, and other molecules, secreted by various cells into physiological fluids. EV-mediated transfer of biomolecules is a critical component of a variety of physiological and pathological processes. Potential applications of EVs in novel diagnostic and therapeutic strategies have brought increasing attention. However, EV research remains highly challenging due to the inherently complex biogenesis of EVs and their vast heterogeneity in size, composition, and origin. There is a need for the establishment of standardized methods that address EV heterogeneity and sources of pre-analytical and analytical variability in EV studies. Here, we review technologies developed for EV isolation and characterization and discuss paths toward standardization in EV research. |*Biomedical Research/methods/standards[MESH] |*Biotechnology/methods/standards[MESH] |*Extracellular Vesicles[MESH] |Animals[MESH] |Bacteria[MESH]Technologies and Standardization in Research on Extracellular Vesicles(epmc).pdf DeepDyve Pubget Overpricing