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10.3390/jcm9061871

http://scihub22266oqcxt.onion/10.3390/jcm9061871
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32560044!7355678!32560044
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suck abstract from ncbi


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pmid32560044      J+Clin+Med 2020 ; 9 (6): ä
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  • Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories #MMPMID32560044
  • Etievant S; Bal A; Escuret V; Brengel-Pesce K; Bouscambert M; Cheynet V; Generenaz L; Oriol G; Destras G; Billaud G; Josset L; Frobert E; Morfin F; Gaymard A
  • J Clin Med 2020[Jun]; 9 (6): ä PMID32560044show ga
  • A reliable diagnostic assay is crucial to early detect new COVID-19 cases and limit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission. Since the onset of the COVID-19 pandemic, the World Health Organization has published several diagnostic molecular approaches developed by referral laboratories, including Charite (Germany), HKU (Hong Kong), China CDC (China), US CDC (United States), and Institut Pasteur, Paris (France). We aimed to compare the sensitivity and specificity of these different RT-PCR assays using SARS-CoV-2 cell culture supernatants and clinical respiratory samples. Overall, the different RT-PCR assays performed well for SARS-CoV-2 detection and were all specific except the N Charite (Germany), and N2 US CDC (United States) assays. RdRp Institut Pasteur (IP2, IP4), N China CDC, and N1 US CDC were found to be the most sensitive assays. The data presented herein are of prime importance to facilitate the equipment choice of diagnostic laboratories, as well as for the development of marketed tests.
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