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10.1371/journal.ppat.1008647 http://scihub22266oqcxt.onion/10.1371/journal.ppat.1008647 32559251!7329128!32559251     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+Pathog 2020 ; 16 (6): e1008647 Nephropedia Template TP {{tp|p=32559251|t=2020. Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology |pdf=|usr=}}{{32559251}} gab.com Text Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology JO: PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=32559251 #FOAvip A trimeric glycoprotein complex on the surface of human cytomegalovirus (HCMV) binds to platelet-derived growth factor (PDGF) receptor alpha (PDGFRalpha) to mediate host cell recognition and fusion of the viral and cellular membranes. Soluble PDGFRalpha potently neutralizes HCMV in tissue culture, and its potential use as an antiviral therapeutic has the benefit that any escape mutants will likely be attenuated. However, PDGFRalpha binds multiple PDGF ligands in the human body as part of developmental programs in embryogenesis and continuing through adulthood. Any therapies with soluble receptor therefore come with serious efficacy and safety concerns, especially for the treatment of congenital HCMV. Soluble virus receptors that are orthogonal to human biology might resolve these concerns. This engineering problem is solved by deep mutational scanning on the D2-D3 domains of PDGFRalpha to identify variants that maintain interactions with the HCMV glycoprotein trimer in the presence of competing PDGF ligands. Competition by PDGFs is conformation-dependent, whereas HCMV trimer binding is independent of proper D2-D3 conformation, and many mutations at the receptor-PDGF interface are suitable for functionally separating trimer from PDGF interactions. Purified soluble PDGFRalpha carrying a targeted mutation succeeded in displaying wild type affinity for HCMV trimer with a simultaneous loss of PDGF binding, and neutralizes trimer-only and trimer/pentamer-expressing HCMV strains infecting fibroblasts or epithelial cells. Overall, this work makes important progress in the realization of soluble HCMV receptors for clinical application. Twit Text FOAVip Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology ????PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=32559251 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32559251 #FOAvip English Wikipedia <ref>{{Cite pmid|32559251}}</ref> Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology #MMPMID32559251 Park J; Gill KS; Aghajani AA; Heredia JD; Choi H; Oberstein A; Procko E PLoS Pathog 2020[Jun]; 16 (6): e1008647 PMID32559251show ga A trimeric glycoprotein complex on the surface of human cytomegalovirus (HCMV) binds to platelet-derived growth factor (PDGF) receptor alpha (PDGFRalpha) to mediate host cell recognition and fusion of the viral and cellular membranes. Soluble PDGFRalpha potently neutralizes HCMV in tissue culture, and its potential use as an antiviral therapeutic has the benefit that any escape mutants will likely be attenuated. However, PDGFRalpha binds multiple PDGF ligands in the human body as part of developmental programs in embryogenesis and continuing through adulthood. Any therapies with soluble receptor therefore come with serious efficacy and safety concerns, especially for the treatment of congenital HCMV. Soluble virus receptors that are orthogonal to human biology might resolve these concerns. This engineering problem is solved by deep mutational scanning on the D2-D3 domains of PDGFRalpha to identify variants that maintain interactions with the HCMV glycoprotein trimer in the presence of competing PDGF ligands. Competition by PDGFs is conformation-dependent, whereas HCMV trimer binding is independent of proper D2-D3 conformation, and many mutations at the receptor-PDGF interface are suitable for functionally separating trimer from PDGF interactions. Purified soluble PDGFRalpha carrying a targeted mutation succeeded in displaying wild type affinity for HCMV trimer with a simultaneous loss of PDGF binding, and neutralizes trimer-only and trimer/pentamer-expressing HCMV strains infecting fibroblasts or epithelial cells. Overall, this work makes important progress in the realization of soluble HCMV receptors for clinical application. |*Cytomegalovirus Infections/genetics/metabolism[MESH] |*Cytomegalovirus/chemistry/genetics/metabolism[MESH] |*Protein Structure, Quaternary[MESH] |*Receptors, Virus/chemistry/genetics/metabolism[MESH] |Cell Line[MESH] |Epithelial Cells/metabolism/pathology/virology[MESH] |Fibroblasts/metabolism/pathology/virology[MESH] |Humans[MESH] |Mutation[MESH] |Protein Domains[MESH]Engineered receptors for human cytomegalovirus that are orthogonal to (epmc).pdf DeepDyve Pubget Overpricing