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  • Isolation, Sequence, Infectivity, and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2 #MMPMID32558639
  • Banerjee A; Nasir JA; Budylowski P; Yip L; Aftanas P; Christie N; Ghalami A; Baid K; Raphenya AR; Hirota JA; Miller MS; McGeer AJ; Ostrowski M; Kozak RA; McArthur AG; Mossman K; Mubareka S
  • Emerg Infect Dis 2020[Sep]; 26 (9): 2054-2063 PMID32558639show ga
  • Since its emergence in Wuhan, China, in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected approximately 6 million persons worldwide. As SARS-CoV-2 spreads across the planet, we explored the range of human cells that can be infected by this virus. We isolated SARS-CoV-2 from 2 infected patients in Toronto, Canada; determined the genomic sequences; and identified single-nucleotide changes in representative populations of our virus stocks. We also tested a wide range of human immune cells for productive infection with SARS-CoV-2. We confirm that human primary peripheral blood mononuclear cells are not permissive for SARS-CoV-2. As SARS-CoV-2 continues to spread globally, it is essential to monitor single-nucleotide polymorphisms in the virus and to continue to isolate circulating viruses to determine viral genotype and phenotype by using in vitro and in vivo infection models.
  • |*Betacoronavirus/genetics/isolation & purification/physiology[MESH]
  • |COVID-19[MESH]
  • |Coronavirus Infections/*virology[MESH]
  • |DNA, Viral/genetics/isolation & purification[MESH]
  • |Genotype[MESH]
  • |Humans[MESH]
  • |Kinetics[MESH]
  • |Leukocytes, Mononuclear/*virology[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*virology[MESH]
  • |Polymorphism, Single Nucleotide[MESH]
  • |SARS-CoV-2[MESH]
  • |Virus Replication/*genetics[MESH]
  • |Whole Genome Sequencing[MESH]

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  • suck abstract from ncbi

    2054 9.26 2020