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10.7554/eLife.56998 http://scihub22266oqcxt.onion/10.7554/eLife.56998 32553109!7351490!32553109     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Elife 2020 ; 9 (ä): ä Nephropedia Template TP {{tp|p=32553109|t=2020. Droplet-based high-throughput cultivation for accurate screening of antibiotic resistant gut microbes |pdf=|usr=}}{{32553109}} gab.com Text Droplet-based high-throughput cultivation for accurate screening of antibiotic resistant gut microbes JO: Elife http://www.kidney.de/pget.php?&tw=1&pmid=32553109 #FOAvip Traditional cultivation approaches in microbiology are labor-intensive, low-throughput, and yield biased sampling of environmental microbes due to ecological and evolutionary factors. New strategies are needed for ample representation of rare taxa and slow-growers that are often outcompeted by fast-growers in cultivation experiments. Here we describe a microfluidic platform that anaerobically isolates and cultivates microbial cells in millions of picoliter droplets and automatically sorts them based on colony density to enhance slow-growing organisms. We applied our strategy to a fecal microbiota transplant (FMT) donor stool using multiple growth media, and found significant increase in taxonomic richness and larger representation of rare and clinically relevant taxa among droplet-grown cells compared to conventional plates. Furthermore, screening the FMT donor stool for antibiotic resistance revealed 21 populations that evaded detection in plate-based assessment of antibiotic resistance. Our method improves cultivation-based surveys of diverse microbiomes to gain deeper insights into microbial functioning and lifestyles. Twit Text FOAVip Droplet-based high-throughput cultivation for accurate screening of antibiotic resistant gut microbes ????Elife http://www.kidney.de/pget.php?&tw=1&pmid=32553109 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32553109 #FOAvip English Wikipedia <ref>{{Cite pmid|32553109}}</ref> Droplet-based high-throughput cultivation for accurate screening of antibiotic resistant gut microbes #MMPMID32553109 Watterson WJ; Tanyeri M; Watson AR; Cham CM; Shan Y; Chang EB; Eren AM; Tay S Elife 2020[Jun]; 9 (ä): ä PMID32553109show ga Traditional cultivation approaches in microbiology are labor-intensive, low-throughput, and yield biased sampling of environmental microbes due to ecological and evolutionary factors. New strategies are needed for ample representation of rare taxa and slow-growers that are often outcompeted by fast-growers in cultivation experiments. Here we describe a microfluidic platform that anaerobically isolates and cultivates microbial cells in millions of picoliter droplets and automatically sorts them based on colony density to enhance slow-growing organisms. We applied our strategy to a fecal microbiota transplant (FMT) donor stool using multiple growth media, and found significant increase in taxonomic richness and larger representation of rare and clinically relevant taxa among droplet-grown cells compared to conventional plates. Furthermore, screening the FMT donor stool for antibiotic resistance revealed 21 populations that evaded detection in plate-based assessment of antibiotic resistance. Our method improves cultivation-based surveys of diverse microbiomes to gain deeper insights into microbial functioning and lifestyles. |*Drug Resistance, Bacterial[MESH] |*Gastrointestinal Microbiome[MESH] |Bacteria/*drug effects[MESH] |Bacteriological Techniques/instrumentation/*methods[MESH]Droplet-based high-throughput cultivation for accurate screening of an(epmc).pdf DeepDyve Pubget Overpricing