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10.7717/peerj.9278 http://scihub22266oqcxt.onion/10.7717/peerj.9278 32547882!7275676!32547882     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32547882&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       PeerJ 2020 ; 8 (?): e9278 Nephropedia Template TP {{tp|p=32547882|t=2020. Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2 |pdf=|usr=}}{{32547882}} gab.com Text Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2 JO: PeerJ http://www.kidney.de/pget.php?&tw=1&pmid=32547882 #FOAvip BACKGROUND: Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid. METHODS: A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. RESULTS: This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings. Twit Text FOAVip Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2 ????PeerJ http://www.kidney.de/pget.php?&tw=1&pmid=32547882 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32547882 #FOAvip English Wikipedia <ref>{{Cite pmid|32547882}}</ref> Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2 #MMPMID32547882 Lau YL; Ismail I; Mustapa NI; Lai MY; Tuan Soh TS; Hassan A; Peariasamy KM; Lee YL; Chong YM; Sam IC; Goh PP PeerJ 2020[]; 8 (?): e9278 PMID32547882show ga BACKGROUND: Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid. METHODS: A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. RESULTS: This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings. ?Real-time reverse transcription loop-mediated isothermal amplification(epmc).pdf DeepDyve Pubget Overpricing