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10.1038/s41467-020-16961-8 http://scihub22266oqcxt.onion/10.1038/s41467-020-16961-8 32546731!7297798!32546731     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2020 ; 11 (1): 3061 Nephropedia Template TP {{tp|p=32546731|t=2020. High-throughput interrogation of programmed ribosomal frameshifting in human cells |pdf=|usr=}}{{32546731}} gab.com Text High-throughput interrogation of programmed ribosomal frameshifting in human cells JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=32546731 #FOAvip Programmed ribosomal frameshifting (PRF) is the controlled slippage of the translating ribosome to an alternative frame. This process is widely employed by human viruses such as HIV and SARS coronavirus and is critical for their replication. Here, we developed a high-throughput approach to assess the frameshifting potential of a sequence. We designed and tested >12,000 sequences based on 15 viral and human PRF events, allowing us to systematically dissect the rules governing ribosomal frameshifting and discover novel regulatory inputs based on amino acid properties and tRNA availability. We assessed the natural variation in HIV gag-pol frameshifting rates by testing >500 clinical isolates and identified subtype-specific differences and associations between viral load in patients and the optimality of PRF rates. We devised computational models that accurately predict frameshifting potential and frameshifting rates, including subtle differences between HIV isolates. This approach can contribute to the development of antiviral agents targeting PRF. Twit Text FOAVip High-throughput interrogation of programmed ribosomal frameshifting in human cells ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=32546731 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32546731 #FOAvip English Wikipedia <ref>{{Cite pmid|32546731}}</ref> High-throughput interrogation of programmed ribosomal frameshifting in human cells #MMPMID32546731 Mikl M; Pilpel Y; Segal E Nat Commun 2020[Jun]; 11 (1): 3061 PMID32546731show ga Programmed ribosomal frameshifting (PRF) is the controlled slippage of the translating ribosome to an alternative frame. This process is widely employed by human viruses such as HIV and SARS coronavirus and is critical for their replication. Here, we developed a high-throughput approach to assess the frameshifting potential of a sequence. We designed and tested >12,000 sequences based on 15 viral and human PRF events, allowing us to systematically dissect the rules governing ribosomal frameshifting and discover novel regulatory inputs based on amino acid properties and tRNA availability. We assessed the natural variation in HIV gag-pol frameshifting rates by testing >500 clinical isolates and identified subtype-specific differences and associations between viral load in patients and the optimality of PRF rates. We devised computational models that accurately predict frameshifting potential and frameshifting rates, including subtle differences between HIV isolates. This approach can contribute to the development of antiviral agents targeting PRF. |*Frameshifting, Ribosomal[MESH] |Fusion Proteins, gag-pol/genetics[MESH] |Genetic Variation[MESH] |Green Fluorescent Proteins/genetics[MESH] |HIV-1/genetics[MESH] |High-Throughput Nucleotide Sequencing/*methods[MESH] |Humans[MESH] |K562 Cells[MESH] |Luminescent Proteins/genetics[MESH] |Protein Biosynthesis[MESH] |RNA, Transfer/genetics[MESH]High-throughput interrogation of programmed ribosomal frameshifting in(epmc).pdf DeepDyve Pubget Overpricing