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10.18632/aging.103416 http://scihub22266oqcxt.onion/10.18632/aging.103416 32544884!7343451!32544884     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Aging+(Albany+NY) 2020 ; 12 (12): 11263-11276 Nephropedia Template TP {{tp|p=32544884|t=2020. De novo design of protein peptides to block association of the SARS-CoV-2 spike protein with human ACE2 |pdf=|usr=}}{{32544884}} gab.com Text De novo design of protein peptides to block association of the SARS-CoV-2 spike protein with human ACE2 JO: Aging (Albany NY) http://www.kidney.de/pget.php?&tw=1&pmid=32544884 #FOAvip The outbreak of COVID-19 has now become a global pandemic that has severely impacted lives and economic stability. There is, however, no effective antiviral drug that can be used to treat COVID-19 to date. Built on the fact that SARS-CoV-2 initiates its entry into human cells by the receptor binding domain (RBD) of its spike protein binding to the angiotensin-converting enzyme 2 (hACE2), we extended a recently developed approach, EvoDesign, to design multiple peptide sequences that can competitively bind to the SARS-CoV-2 RBD to inhibit the virus from entering human cells. The protocol starts with the construction of a hybrid peptidic scaffold by linking two fragments grafted from the interface of the hACE2 protein (a.a. 22-44 and 351-357) with a linker glycine, which is followed by the redesign and refinement simulations of the peptide sequence to optimize its binding affinity to the interface of the SARS-CoV-2 RBD. The binding experiment analyses showed that the designed peptides exhibited a significantly stronger binding potency to hACE2 than the wild-type hACE2 receptor (with -53.35 vs. -46.46 EvoEF2 energy unit scores for the top designed and wild-type peptides, respectively). This study demonstrates a new avenue to utilize computationally designed peptide motifs to treat the COVID-19 disease by blocking the critical spike-RBD and hACE2 interactions. Twit Text FOAVip De novo design of protein peptides to block association of the SARS-CoV-2 spike protein with human ACE2 ????Aging (Albany NY) http://www.kidney.de/pget.php?&tw=1&pmid=32544884 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32544884 #FOAvip English Wikipedia <ref>{{Cite pmid|32544884}}</ref> De novo design of protein peptides to block association of the SARS-CoV-2 spike protein with human ACE2 #MMPMID32544884 Huang X; Pearce R; Zhang Y Aging (Albany NY) 2020[Jun]; 12 (12): 11263-11276 PMID32544884show ga The outbreak of COVID-19 has now become a global pandemic that has severely impacted lives and economic stability. There is, however, no effective antiviral drug that can be used to treat COVID-19 to date. Built on the fact that SARS-CoV-2 initiates its entry into human cells by the receptor binding domain (RBD) of its spike protein binding to the angiotensin-converting enzyme 2 (hACE2), we extended a recently developed approach, EvoDesign, to design multiple peptide sequences that can competitively bind to the SARS-CoV-2 RBD to inhibit the virus from entering human cells. The protocol starts with the construction of a hybrid peptidic scaffold by linking two fragments grafted from the interface of the hACE2 protein (a.a. 22-44 and 351-357) with a linker glycine, which is followed by the redesign and refinement simulations of the peptide sequence to optimize its binding affinity to the interface of the SARS-CoV-2 RBD. The binding experiment analyses showed that the designed peptides exhibited a significantly stronger binding potency to hACE2 than the wild-type hACE2 receptor (with -53.35 vs. -46.46 EvoEF2 energy unit scores for the top designed and wild-type peptides, respectively). This study demonstrates a new avenue to utilize computationally designed peptide motifs to treat the COVID-19 disease by blocking the critical spike-RBD and hACE2 interactions. |Amino Acid Sequence[MESH] |Angiotensin-Converting Enzyme 2[MESH] |Antiviral Agents[MESH] |Binding Sites[MESH] |COVID-19[MESH] |Coronavirus Infections/*drug therapy[MESH] |Drug Design[MESH] |Evolution, Molecular[MESH] |Humans[MESH] |Models, Molecular[MESH] |Pandemics[MESH] |Peptides/*chemical synthesis/*pharmacology[MESH] |Peptidyl-Dipeptidase A/*physiology[MESH] |Pneumonia, Viral/*drug therapy[MESH] |Protein Binding[MESH] |Protein Conformation[MESH] |Spike Glycoprotein, Coronavirus/*physiology[MESH]De novo design of protein peptides to block association of the SARS-Co(epmc).pdf DeepDyve Pubget Overpricing