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10.1002/jmv.26187 http://scihub22266oqcxt.onion/10.1002/jmv.26187 32543753!7323082!32543753     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2020 ; 92 (11): 2777-2784 Nephropedia Template TP {{tp|p=32543753|t=2020. Comparison and application of different immunoassay methods for the detection of SARS-CoV-2 |pdf=|usr=}}{{32543753}} gab.com Text Comparison and application of different immunoassay methods for the detection of SARS-CoV-2 JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=32543753 #FOAvip The detection data of IgM and IgG antibodies in 169 patients with coronavirus disease-2019 (COVID-19) were analyzed to evaluate differences in clinical performance between the colloidal gold method and chemiluminescence method. In this study, chemiluminescence detection of IgM antibody showed a positive conversion earlier (about 1-2 days earlier), positive conversion rates higher in different stages of disease, and a trend of declining positive rate later than colloidal gold method. For IgG antibody, the chemiluminescence method showed a positive conversion earlier and the positive rate climbing more quickly than the colloidal gold method. No obvious negative-converting tendency of IgG detection was observed within 35 days after the onset of disease. Although colloidal gold method is generally less sensitive than chemiluminescence method, it shows advantages of shorter turn-around time, more simple procedure, and no special equipment required. The two methodologies can be chosen according to different laboratory conditions. A reasonable understanding of the performance of reagents with different methodologies can help in clinical disease diagnosis effectively and assist in the diagnosis of the progression of COVID-19, for which the dynamic changes of antibody will provide reliable evidence. Twit Text FOAVip Comparison and application of different immunoassay methods for the detection of SARS-CoV-2 ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=32543753 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32543753 #FOAvip English Wikipedia <ref>{{Cite pmid|32543753}}</ref> Comparison and application of different immunoassay methods for the detection of SARS-CoV-2 #MMPMID32543753 He J; Hu P; Gao Y; Zheng S; Xu C; Liu R; Fang L; Li R; Han C; An J; Dong J; Deng G; Sun L; Lv Y J Med Virol 2020[Nov]; 92 (11): 2777-2784 PMID32543753show ga The detection data of IgM and IgG antibodies in 169 patients with coronavirus disease-2019 (COVID-19) were analyzed to evaluate differences in clinical performance between the colloidal gold method and chemiluminescence method. In this study, chemiluminescence detection of IgM antibody showed a positive conversion earlier (about 1-2 days earlier), positive conversion rates higher in different stages of disease, and a trend of declining positive rate later than colloidal gold method. For IgG antibody, the chemiluminescence method showed a positive conversion earlier and the positive rate climbing more quickly than the colloidal gold method. No obvious negative-converting tendency of IgG detection was observed within 35 days after the onset of disease. Although colloidal gold method is generally less sensitive than chemiluminescence method, it shows advantages of shorter turn-around time, more simple procedure, and no special equipment required. The two methodologies can be chosen according to different laboratory conditions. A reasonable understanding of the performance of reagents with different methodologies can help in clinical disease diagnosis effectively and assist in the diagnosis of the progression of COVID-19, for which the dynamic changes of antibody will provide reliable evidence. |Adolescent[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |Antibodies, Viral/*analysis[MESH] |COVID-19/*diagnosis/immunology[MESH] |Clinical Trials as Topic[MESH] |Female[MESH] |Gold Colloid[MESH] |Humans[MESH] |Immunoassay/*methods[MESH] |Immunoglobulin G/analysis[MESH] |Immunoglobulin M/analysis[MESH] |Luminescence[MESH] |Male[MESH] |Middle Aged[MESH]Comparison and application of different immunoassay methods for the de(epmc).pdf DeepDyve Pubget Overpricing