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10.1080/22221751.2020.1782271

http://scihub22266oqcxt.onion/10.1080/22221751.2020.1782271
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suck abstract from ncbi


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pmid32543353      Emerg+Microbes+Infect 2020 ; 9 (1): 1457-1466
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  • SARS-CoV-2 genomic surveillance in Taiwan revealed novel ORF8-deletion mutant and clade possibly associated with infections in Middle East #MMPMID32543353
  • Gong YN; Tsao KC; Hsiao MJ; Huang CG; Huang PN; Huang PW; Lee KM; Liu YC; Yang SL; Kuo RL; Chen KF; Liu YC; Huang SY; Huang HI; Liu MT; Yang JR; Chiu CH; Yang CT; Chen GW; Shih SR
  • Emerg Microbes Infect 2020[Dec]; 9 (1): 1457-1466 PMID32543353show ga
  • Taiwan experienced two waves of imported infections with Coronavirus Disease 2019 (COVID-19). This study aimed at investigating the genomic variation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Taiwan and compared their evolutionary trajectories with the global strains. We performed culture and full-genome sequencing of SARS-CoV-2 strains followed by phylogenetic analysis. A 382-nucleotides deletion in open reading frame 8 (ORF8) was found in a Taiwanese strain isolated from a patient on February 4, 2020 who had a travel history to Wuhan. Patients in the first wave also included several sporadic, local transmission cases. Genomes of 5 strains sequenced from clustered infections were classified into a new clade with ORF1ab-V378I mutation, in addition to 3 dominant clades ORF8-L84S, ORF3a-G251V and S-D614G. This highlighted clade also included some strains isolated from patients who had a travel history to Turkey and Iran. The second wave mostly resulted from patients who had a travel history to Europe and Americas. All Taiwanese viruses were classified into various clades. Genomic surveillance of SARS-CoV-2 in Taiwan revealed a new ORF8-deletion mutant and a virus clade that may be associated with infections in the Middle East, which contributed to a better understanding of the global SARS-CoV-2 transmission dynamics.
  • |*Genome, Viral[MESH]
  • |Animals[MESH]
  • |Betacoronavirus/classification/*genetics/isolation & purification[MESH]
  • |COVID-19[MESH]
  • |Cell Line[MESH]
  • |Chlorocebus aethiops[MESH]
  • |Coronavirus Infections/*virology[MESH]
  • |Haemophilus parainfluenzae/isolation & purification[MESH]
  • |Humans[MESH]
  • |Middle East[MESH]
  • |Open Reading Frames[MESH]
  • |Pandemics[MESH]
  • |Phylogeny[MESH]
  • |Pneumonia, Viral/*virology[MESH]
  • |RNA, Viral[MESH]
  • |SARS-CoV-2[MESH]
  • |Sequence Deletion[MESH]
  • |Taiwan[MESH]
  • |Travel[MESH]
  • |Vero Cells[MESH]
  • |Virus Cultivation[MESH]


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