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10.1016/j.cell.2020.05.042 http://scihub22266oqcxt.onion/10.1016/j.cell.2020.05.042 32526206!7250779!32526206     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell 2020 ; 182 (2): 429-446.e14 Nephropedia Template TP {{tp|p=32526206|t=2020. SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract |pdf=|usr=}}{{32526206}} gab.com Text SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=32526206 #FOAvip The mode of acquisition and causes for the variable clinical spectrum of coronavirus disease 2019 (COVID-19) remain unknown. We utilized a reverse genetics system to generate a GFP reporter virus to explore severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pathogenesis and a luciferase reporter virus to demonstrate sera collected from SARS and COVID-19 patients exhibited limited cross-CoV neutralization. High-sensitivity RNA in situ mapping revealed the highest angiotensin-converting enzyme 2 (ACE2) expression in the nose with decreasing expression throughout the lower respiratory tract, paralleled by a striking gradient of SARS-CoV-2 infection in proximal (high) versus distal (low) pulmonary epithelial cultures. COVID-19 autopsied lung studies identified focal disease and, congruent with culture data, SARS-CoV-2-infected ciliated and type 2 pneumocyte cells in airway and alveolar regions, respectively. These findings highlight the nasal susceptibility to SARS-CoV-2 with likely subsequent aspiration-mediated virus seeding to the lung in SARS-CoV-2 pathogenesis. These reagents provide a foundation for investigations into virus-host interactions in protective immunity, host susceptibility, and virus pathogenesis. Twit Text FOAVip SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=32526206 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32526206 #FOAvip English Wikipedia <ref>{{Cite pmid|32526206}}</ref> SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract #MMPMID32526206 Hou YJ; Okuda K; Edwards CE; Martinez DR; Asakura T; Dinnon KH 3rd; Kato T; Lee RE; Yount BL; Mascenik TM; Chen G; Olivier KN; Ghio A; Tse LV; Leist SR; Gralinski LE; Schafer A; Dang H; Gilmore R; Nakano S; Sun L; Fulcher ML; Livraghi-Butrico A; Nicely NI; Cameron M; Cameron C; Kelvin DJ; de Silva A; Margolis DM; Markmann A; Bartelt L; Zumwalt R; Martinez FJ; Salvatore SP; Borczuk A; Tata PR; Sontake V; Kimple A; Jaspers I; O'Neal WK; Randell SH; Boucher RC; Baric RS Cell 2020[Jul]; 182 (2): 429-446.e14 PMID32526206show ga The mode of acquisition and causes for the variable clinical spectrum of coronavirus disease 2019 (COVID-19) remain unknown. We utilized a reverse genetics system to generate a GFP reporter virus to explore severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pathogenesis and a luciferase reporter virus to demonstrate sera collected from SARS and COVID-19 patients exhibited limited cross-CoV neutralization. High-sensitivity RNA in situ mapping revealed the highest angiotensin-converting enzyme 2 (ACE2) expression in the nose with decreasing expression throughout the lower respiratory tract, paralleled by a striking gradient of SARS-CoV-2 infection in proximal (high) versus distal (low) pulmonary epithelial cultures. COVID-19 autopsied lung studies identified focal disease and, congruent with culture data, SARS-CoV-2-infected ciliated and type 2 pneumocyte cells in airway and alveolar regions, respectively. These findings highlight the nasal susceptibility to SARS-CoV-2 with likely subsequent aspiration-mediated virus seeding to the lung in SARS-CoV-2 pathogenesis. These reagents provide a foundation for investigations into virus-host interactions in protective immunity, host susceptibility, and virus pathogenesis. |Aged[MESH] |Angiotensin-Converting Enzyme 2[MESH] |Animals[MESH] |Antibodies, Monoclonal/immunology[MESH] |Antibodies, Neutralizing/immunology[MESH] |Betacoronavirus/*genetics/immunology/pathogenicity[MESH] |COVID-19[MESH] |COVID-19 Serotherapy[MESH] |Cell Line[MESH] |Cells, Cultured[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Infections/immunology/*pathology/therapy/*virology[MESH] |Cystic Fibrosis/pathology[MESH] |DNA, Recombinant[MESH] |Female[MESH] |Furin/metabolism[MESH] |Humans[MESH] |Immunization, Passive[MESH] |Lung/metabolism/pathology/virology[MESH] |Male[MESH] |Middle Aged[MESH] |Nasal Mucosa/metabolism/pathology/virology[MESH] |Pandemics[MESH] |Peptidyl-Dipeptidase A/metabolism[MESH] |Pneumonia, Viral/immunology/*pathology/*virology[MESH] |Respiratory System/pathology/*virology[MESH] |Reverse Genetics/*methods[MESH] |SARS-CoV-2[MESH] |Serine Endopeptidases/metabolism[MESH] |Vero Cells[MESH] |Virulence[MESH]SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in t(epmc).pdf DeepDyve Pubget Overpricing