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10.3390/v12060624 http://scihub22266oqcxt.onion/10.3390/v12060624 32521706!7354533!32521706     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2020 ; 12 (6): ä Nephropedia Template TP {{tp|p=32521706|t=2020. Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples |pdf=|usr=}}{{32521706}} gab.com Text Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32521706 #FOAvip Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 minute contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective. Twit Text FOAVip Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32521706 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32521706 #FOAvip English Wikipedia <ref>{{Cite pmid|32521706}}</ref> Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples #MMPMID32521706 Pastorino B; Touret F; Gilles M; Luciani L; de Lamballerie X; Charrel RN Viruses 2020[Jun]; 12 (6): ä PMID32521706show ga Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 minute contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective. |Animals[MESH] |Betacoronavirus/*drug effects/genetics/isolation & purification/physiology[MESH] |COVID-19[MESH] |Cell Culture Techniques/methods[MESH] |Chlorocebus aethiops[MESH] |Containment of Biohazards/methods/standards[MESH] |Coronavirus Infections/*virology[MESH] |Humans[MESH] |Nasopharynx/virology[MESH] |Pandemics[MESH] |Pneumonia, Viral/*virology[MESH] |RNA, Viral/isolation & purification[MESH] |SARS-CoV-2[MESH] |Specimen Handling/methods[MESH] |Vero Cells[MESH] |Viral Load/methods[MESH]Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectiou(epmc).pdf DeepDyve Pubget Overpricing