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10.1016/j.bsheal.2019.12.003 http://scihub22266oqcxt.onion/10.1016/j.bsheal.2019.12.003 32501448!7148593!32501448     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosaf+Health 2019 ; 1 (3): 150-154 Nephropedia Template TP {{tp|p=32501448|t=2019. Identification of Histoplasma causing an unexplained disease cluster in Matthews Ridge, Guyana |pdf=|usr=}}{{32501448}} gab.com Text Identification of Histoplasma causing an unexplained disease cluster in Matthews Ridge, Guyana JO: Biosaf Health http://www.kidney.de/pget.php?&tw=1&pmid=32501448 #FOAvip Here, we report the identification of Histoplasma causing an unexplained disease cluster in Matthews Ridge, Guyana. In March 2019, 14 employees of Chongqing Bosai Mining Company, China, working in a manganese mining of Guyana, had unexplained fever, and two of them died. We obtained lung and brain tissues as well as the blood samples from the two deceased cases (patient No. 1 and 2), and bronchoscopy lavages and cerebrospinal fluid samples from one severe case (patient No. 3), respectively. All samples were tested by pathological examination, high-throughput sequencing, and real-time PCR. Pathological detection showed the presence of spore-like structures in the lung tissue of patient No. 1, indicating a fungal infection in this patient. Nanopore sequencing identified the existing of H. capsulatum in the lung tissue sample within 13 h. Next-generation sequencing identified specific fragments of H. capsulatum in all of the samples tested (lung, brain and blood serum from the deceased cases, and plasma from the severe case). Real-time PCR assays did not reveal any viral infection related to transmission from bat feces. We conclude that H. capsulatum was the causative pathogen of this disease cluster based on epidemiologic, clinical, pathological and nucleic acid evidence. Twit Text FOAVip Identification of Histoplasma causing an unexplained disease cluster in Matthews Ridge, Guyana ????Biosaf Health http://www.kidney.de/pget.php?&tw=1&pmid=32501448 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32501448 #FOAvip English Wikipedia <ref>{{Cite pmid|32501448}}</ref> Identification of Histoplasma causing an unexplained disease cluster in Matthews Ridge, Guyana #MMPMID32501448 Wang J; Zhou W; Ling H; Dong X; Zhang Y; Li J; Zhang Y; Song J; Liu WJ; Li Y; Zhang R; Zhen W; Cai K; Zhu S; Wang D; Xiao J; Tong Y; Liu W; Song L; Wu W; Liu Y; Zhao X; Wang R; Ye S; Wang J; Lu R; Huang B; Ye F; Lei W; Gao R; Shi Q; Chen C; Han J; Xu W; Gao GF; Ma X; Wu G Biosaf Health 2019[Dec]; 1 (3): 150-154 PMID32501448show ga Here, we report the identification of Histoplasma causing an unexplained disease cluster in Matthews Ridge, Guyana. In March 2019, 14 employees of Chongqing Bosai Mining Company, China, working in a manganese mining of Guyana, had unexplained fever, and two of them died. We obtained lung and brain tissues as well as the blood samples from the two deceased cases (patient No. 1 and 2), and bronchoscopy lavages and cerebrospinal fluid samples from one severe case (patient No. 3), respectively. All samples were tested by pathological examination, high-throughput sequencing, and real-time PCR. Pathological detection showed the presence of spore-like structures in the lung tissue of patient No. 1, indicating a fungal infection in this patient. Nanopore sequencing identified the existing of H. capsulatum in the lung tissue sample within 13 h. Next-generation sequencing identified specific fragments of H. capsulatum in all of the samples tested (lung, brain and blood serum from the deceased cases, and plasma from the severe case). Real-time PCR assays did not reveal any viral infection related to transmission from bat feces. We conclude that H. capsulatum was the causative pathogen of this disease cluster based on epidemiologic, clinical, pathological and nucleic acid evidence. äIdentification of Histoplasma causing an unexplained disease cluster i(epmc).pdf DeepDyve Pubget Overpricing