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10.1016/j.ijid.2020.05.099 http://scihub22266oqcxt.onion/10.1016/j.ijid.2020.05.099 32492531!7833505!32492531     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Infect+Dis 2020 ; 97 (ä): 66-68 Nephropedia Template TP {{tp|p=32492531|t=2020. Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples |pdf=|usr=}}{{32492531}} gab.com Text Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples JO: Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32492531 #FOAvip OBJECTIVES: The gold-standard COVID-19 diagnosis relies on detecting SARS-CoV-2 using RNA purification and one-step retrotranscription and quantitative PCR (RT-qPCR). Based on the urgent need for high-throughput screening, we tested the performance of three alternative, simple and affordable protocols to rapidly detect SARS-CoV-2, bypassing the long and tedious RNA extraction step and reducing the time to viral detection. METHODS: We evaluated three methods based on direct nasopharyngeal swab viral transmission medium (VTM) heating before the RT-qPCR: a) direct without additives; b) in a formamide-EDTA (FAE) buffer, c) in a RNAsnap(TM) buffer. RESULTS: Although with a delay in cycle threshold compared to the gold-standard, we found consistent results in nasopharyngeal swab samples that were subject to a direct 70 degrees C incubation for 10 min. CONCLUSIONS: Our findings provide valuable options to overcome any supply chain issue and help to increase the throughput of diagnostic tests, thereby complementing standard diagnosis. Twit Text FOAVip Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples ????Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32492531 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32492531 #FOAvip English Wikipedia <ref>{{Cite pmid|32492531}}</ref> Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples #MMPMID32492531 Alcoba-Florez J; Gonzalez-Montelongo R; Inigo-Campos A; de Artola DG; Gil-Campesino H; The Microbiology Technical Support Team; Ciuffreda L; Valenzuela-Fernandez A; Flores C Int J Infect Dis 2020[Aug]; 97 (ä): 66-68 PMID32492531show ga OBJECTIVES: The gold-standard COVID-19 diagnosis relies on detecting SARS-CoV-2 using RNA purification and one-step retrotranscription and quantitative PCR (RT-qPCR). Based on the urgent need for high-throughput screening, we tested the performance of three alternative, simple and affordable protocols to rapidly detect SARS-CoV-2, bypassing the long and tedious RNA extraction step and reducing the time to viral detection. METHODS: We evaluated three methods based on direct nasopharyngeal swab viral transmission medium (VTM) heating before the RT-qPCR: a) direct without additives; b) in a formamide-EDTA (FAE) buffer, c) in a RNAsnap(TM) buffer. RESULTS: Although with a delay in cycle threshold compared to the gold-standard, we found consistent results in nasopharyngeal swab samples that were subject to a direct 70 degrees C incubation for 10 min. CONCLUSIONS: Our findings provide valuable options to overcome any supply chain issue and help to increase the throughput of diagnostic tests, thereby complementing standard diagnosis. |Betacoronavirus/*genetics[MESH] |COVID-19[MESH] |Coronavirus Infections/*diagnosis/virology[MESH] |Diagnostic Tests, Routine[MESH] |Hot Temperature[MESH] |Humans[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/virology[MESH] |Real-Time Polymerase Chain Reaction[MESH]Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab (epmc).pdf DeepDyve Pubget Overpricing