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10.1002/jmv.26116 http://scihub22266oqcxt.onion/10.1002/jmv.26116 32492196!7300714!32492196     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2020 ; 92 (10): 2221-2226 Nephropedia Template TP {{tp|p=32492196|t=2020. High-coverage SARS-CoV-2 genome sequences acquired by target capture sequencing |pdf=|usr=}}{{32492196}} gab.com Text High-coverage SARS-CoV-2 genome sequences acquired by target capture sequencing JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=32492196 #FOAvip In this study, we designed a set of SARS-CoV-2 enrichment probes to increase the capacity for sequence-based virus detection and obtain the comprehensive genome sequence at the same time. This universal SARS-CoV-2 enrichment probe set contains 502 120 nt single-stranded DNA biotin-labeled probes designed based on all available SARS-CoV-2 viral sequences and it can be used to enrich for SARS-CoV-2 sequences without prior knowledge of type or subtype. Following the CDC health and safety guidelines, marked enrichment was demonstrated in a virus strain sample from cell culture, three nasopharyngeal swab samples (cycle threshold [C(t) ] values: 32.36, 36.72, and 38.44) from patients diagnosed with COVID-19 (positive control) and four throat swab samples from patients without COVID-19 (negative controls), respectively. Moreover, based on these high-quality sequences, we discuss the heterozygosity and viral expression during coronavirus replication and its phylogenetic relationship with other selected high-quality samples from the Genome Variation Map. Therefore, this universal SARS-CoV-2 enrichment probe system can capture and enrich SARS-CoV-2 viral sequences selectively and effectively in different samples, especially clinical swab samples with a relatively low concentration of viral particles. Twit Text FOAVip High-coverage SARS-CoV-2 genome sequences acquired by target capture sequencing ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=32492196 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32492196 #FOAvip English Wikipedia <ref>{{Cite pmid|32492196}}</ref> High-coverage SARS-CoV-2 genome sequences acquired by target capture sequencing #MMPMID32492196 Wen S; Sun C; Zheng H; Wang L; Zhang H; Zou L; Liu Z; Du P; Xu X; Liang L; Peng X; Zhang W; Wu J; Yang J; Lei B; Zeng G; Ke C; Chen F; Zhang X J Med Virol 2020[Oct]; 92 (10): 2221-2226 PMID32492196show ga In this study, we designed a set of SARS-CoV-2 enrichment probes to increase the capacity for sequence-based virus detection and obtain the comprehensive genome sequence at the same time. This universal SARS-CoV-2 enrichment probe set contains 502 120 nt single-stranded DNA biotin-labeled probes designed based on all available SARS-CoV-2 viral sequences and it can be used to enrich for SARS-CoV-2 sequences without prior knowledge of type or subtype. Following the CDC health and safety guidelines, marked enrichment was demonstrated in a virus strain sample from cell culture, three nasopharyngeal swab samples (cycle threshold [C(t) ] values: 32.36, 36.72, and 38.44) from patients diagnosed with COVID-19 (positive control) and four throat swab samples from patients without COVID-19 (negative controls), respectively. Moreover, based on these high-quality sequences, we discuss the heterozygosity and viral expression during coronavirus replication and its phylogenetic relationship with other selected high-quality samples from the Genome Variation Map. Therefore, this universal SARS-CoV-2 enrichment probe system can capture and enrich SARS-CoV-2 viral sequences selectively and effectively in different samples, especially clinical swab samples with a relatively low concentration of viral particles. |*Genome, Viral[MESH] |Biotin/chemistry[MESH] |COVID-19/*diagnosis/pathology/virology[MESH] |DNA Probes/chemical synthesis/*metabolism[MESH] |DNA, Single-Stranded/*genetics/metabolism[MESH] |Genotype[MESH] |Humans[MESH] |Mutation[MESH] |Nasopharynx/virology[MESH] |Phylogeny[MESH] |Reverse Transcriptase Polymerase Chain Reaction/standards[MESH] |SARS-CoV-2/classification/*genetics/isolation & purification/pathogenicity[MESH] |Sensitivity and Specificity[MESH]High-coverage SARS-CoV-2 genome sequences acquired by target capture s(epmc).pdf DeepDyve Pubget Overpricing