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10.3390/v12060598 http://scihub22266oqcxt.onion/10.3390/v12060598 32486283!7354593!32486283     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\32486283.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Viruses 2020 ; 12 (6): ä Nephropedia Template TP {{tp|p=32486283|t=2020. Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses |pdf=|usr=}}{{32486283}} gab.com Text Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32486283 #FOAvip Single-stranded positive RNA ((+) ssRNA) viruses include several important human pathogens. Some members are responsible for large outbreaks, such as Zika virus, West Nile virus, SARS-CoV, and SARS-CoV-2, while others are endemic, causing an enormous global health burden. Since vaccines or specific treatments are not available for most viral infections, the discovery of direct-acting antivirals (DAA) is an urgent need. Still, the low-throughput nature of and biosafety concerns related to traditional antiviral assays hinders the discovery of new inhibitors. With the advances of reverse genetics, reporter replicon systems have become an alternative tool for the screening of DAAs. Herein, we review decades of the use of (+) ssRNA viruses replicon systems for the discovery of antiviral agents. We summarize different strategies used to develop those systems, as well as highlight some of the most promising inhibitors identified by the method. Despite the genetic alterations introduced, reporter replicons have been shown to be reliable systems for screening and identification of viral replication inhibitors and, therefore, an important tool for the discovery of new DAAs. Twit Text FOAVip Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32486283 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32486283 #FOAvip English Wikipedia <ref>{{Cite pmid|32486283}}</ref> Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses #MMPMID32486283 Fernandes RS; Freire MCLC; Bueno RV; Godoy AS; Gil LHVG; Oliva G Viruses 2020[May]; 12 (6): ä PMID32486283show ga Single-stranded positive RNA ((+) ssRNA) viruses include several important human pathogens. Some members are responsible for large outbreaks, such as Zika virus, West Nile virus, SARS-CoV, and SARS-CoV-2, while others are endemic, causing an enormous global health burden. Since vaccines or specific treatments are not available for most viral infections, the discovery of direct-acting antivirals (DAA) is an urgent need. Still, the low-throughput nature of and biosafety concerns related to traditional antiviral assays hinders the discovery of new inhibitors. With the advances of reverse genetics, reporter replicon systems have become an alternative tool for the screening of DAAs. Herein, we review decades of the use of (+) ssRNA viruses replicon systems for the discovery of antiviral agents. We summarize different strategies used to develop those systems, as well as highlight some of the most promising inhibitors identified by the method. Despite the genetic alterations introduced, reporter replicons have been shown to be reliable systems for screening and identification of viral replication inhibitors and, therefore, an important tool for the discovery of new DAAs. |Animals[MESH] |Antiviral Agents/chemistry/*pharmacology[MESH] |Cell Line[MESH] |Chlorocebus aethiops[MESH] |Cricetinae[MESH] |Drug Discovery/*methods[MESH] |Genes, Reporter/*physiology[MESH] |Humans[MESH] |RNA Viruses/*drug effects/genetics[MESH] |Replicon/*physiology[MESH] |Transfection[MESH]Reporter Replicons for Antiviral Drug Discovery against Positive Singl(epmc).pdf DeepDyve Pubget Overpricing