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10.1016/j.fertnstert.2020.04.024

http://scihub22266oqcxt.onion/10.1016/j.fertnstert.2020.04.024
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suck abstract from ncbi


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pmid32482249      Fertil+Steril 2020 ; 113 (6): 1135-1139
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  • No evidence of severe acute respiratory syndrome-coronavirus 2 in semen of males recovering from coronavirus disease 2019 #MMPMID32482249
  • Pan F; Xiao X; Guo J; Song Y; Li H; Patel DP; Spivak AM; Alukal JP; Zhang X; Xiong C; Li PS; Hotaling JM
  • Fertil Steril 2020[Jun]; 113 (6): 1135-1139 PMID32482249show ga
  • OBJECTIVE: To describe detection of severe acute respiratory syndrome (SARS)-coronavirus 2 (CoV-2) in seminal fluid of patients recovering from coronavirus disease 2019 (COVID-19) and to describe the expression profile of angiotensin-converting enzyme 2 (ACE2) and Transmembrane Serine Protease 2 (TMPRSS2) within the testicle. DESIGN: Observational, cross-sectional study. SETTING: Tertiary referral center. PATIENT(S): Thirty-four adult Chinese males diagnosed with COVID-19 through confirmatory quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) from pharyngeal swab samples. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Identification of SARS-CoV-2 on qRT-PCR of single ejaculated semen samples. Semen quality was not assessed. Expression patterns of ACE2 and TMPRSS2 in the human testis are explored through previously published single-cell transcriptome datasets. RESULT(S): Six patients (19%) demonstrated scrotal discomfort suggestive of viral orchitis around the time of COVID-19 confirmation. Severe acute respiratory syndrome-CoV-2 was not detected in semen after a median of 31 days (interquartile range, 29-36 days) from COVID-19 diagnosis. Single-cell transcriptome analysis demonstrates sparse expression of ACE2 and TMPRSS2, with almost no overlapping gene expression. CONCLUSION(S): Severe acute respiratory syndrome-CoV-2 was not detected in the semen of patients recovering from COVID-19 1 month after COVID-19 diagnosis. Angiotensin-converting enzyme 2-mediated viral entry of SARS-CoV-2 into target host cells is unlikely to occur within the human testicle based on ACE2 and TMPRSS2 expression. The long-term effects of SARS-CoV-2 on male reproductive function remain unknown.
  • |Adolescent[MESH]
  • |Adult[MESH]
  • |Angiotensin-Converting Enzyme 2[MESH]
  • |Betacoronavirus/genetics/*isolation & purification[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Clinical Laboratory Techniques[MESH]
  • |Coronavirus Infections/diagnosis/enzymology/genetics/*virology[MESH]
  • |Cross-Sectional Studies[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Pandemics[MESH]
  • |Peptidyl-Dipeptidase A/genetics[MESH]
  • |Pneumonia, Viral/diagnosis/enzymology/genetics/*virology[MESH]
  • |RNA-Seq[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]
  • |SARS-CoV-2[MESH]
  • |Semen/*virology[MESH]
  • |Serine Endopeptidases/genetics[MESH]
  • |Testis/enzymology/virology[MESH]
  • |Time Factors[MESH]
  • |Transcriptome[MESH]
  • |Virus Internalization[MESH]


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