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10.1080/22221751.2020.1766382

http://scihub22266oqcxt.onion/10.1080/22221751.2020.1766382
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32476607!8284968!32476607
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suck abstract from ncbi


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pmid32476607      Emerg+Microbes+Infect 2020 ; 9 (1): 1096-1101
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  • Delayed specific IgM antibody responses observed among COVID-19 patients with severe progression #MMPMID32476607
  • Shen L; Wang C; Zhao J; Tang X; Shen Y; Lu M; Ding Z; Huang C; Zhang J; Li S; Lan J; Wong G; Zhu Y
  • Emerg Microbes Infect 2020[Dec]; 9 (1): 1096-1101 PMID32476607show ga
  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread rapidly worldwide since it was confirmed as the causative agent of COVID-19. Molecular diagnosis of the disease is typically performed via nucleic acid-based detection of the virus from swabs, sputum or bronchoalveolar lavage fluid (BALF). However, the positive rate from the commonly used specimens (swabs or sputum) was less than 75%. Immunological assays for SARS-CoV-2 are needed to accurately diagnose COVID-19. Sera were collected from patients or healthy people in a local hospital in Xiangyang, Hubei Province, China. The SARS-CoV-2 specific IgM antibodies were then detected using a SARS-CoV-2 IgM colloidal gold immunochromatographic assay (GICA). Results were analysed in combination with sera collection date and clinical information. The GICA was found to be positive with the detected 82.2% (37/45) of RT-qPCR confirmed COVID-19 cases, as well as 32.0% (8/25) of clinically confirmed, RT-qPCR negative patients (4-14 days after symptom onset). Investigation of IgM-negative, RT-qPCR-positive COVID-19 patients showed that half of them developed severe disease. The GICA was found to be a useful test to complement existing PCR-based assays for confirmation of COVID-19, and a delayed specific IgM antibody response was observed among COVID-19 patients with severe progression.
  • |*Antibody Formation[MESH]
  • |Adolescent[MESH]
  • |Adult[MESH]
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |Antibodies, Viral/*blood/immunology[MESH]
  • |Betacoronavirus[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Child[MESH]
  • |China[MESH]
  • |Clinical Laboratory Techniques[MESH]
  • |Coronavirus Infections/*diagnosis/*immunology[MESH]
  • |Disease Progression[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Immunoassay[MESH]
  • |Immunoglobulin M/*blood/immunology[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis/*immunology[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]
  • |SARS-CoV-2[MESH]


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