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10.1002/cpmc.105 http://scihub22266oqcxt.onion/10.1002/cpmc.105 32475066!7300432!32475066     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Curr+Protoc+Microbiol 2020 ; 57 (1): ecpmc105 Nephropedia Template TP {{tp|p=32475066|t=2020. Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS-CoV-2 |pdf=|usr=}}{{32475066}} gab.com Text Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS-CoV-2 JO: Curr Protoc Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=32475066 #FOAvip Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been identified as the causal agent of COronaVIrus Disease-19 (COVID-19), an atypical pneumonia-like syndrome that emerged in December 2019. While SARS-CoV-2 titers can be measured by detection of viral nucleic acid, this method is unable to quantitate infectious virions. Measurement of infectious SARS-CoV-2 can be achieved by tissue culture infectious dose-50 (TCID(50) ), which detects the presence or absence of cytopathic effect in cells infected with serial dilutions of a virus specimen. However, this method only provides a qualitative infectious virus titer. Plaque assays are a quantitative method of measuring infectious SARS-CoV-2 by quantifying the plaques formed in cell culture upon infection with serial dilutions of a virus specimen. As such, plaque assays remain the gold standard in quantifying concentrations of replication-competent lytic virions. Here, we describe two detailed plaque assay protocols to quantify infectious SARS-CoV-2 using different overlay and staining methods. Both methods have several advantages and disadvantages, which can be considered when choosing the procedure best suited for each laboratory. These assays can be used for several research purposes, including titration of virus stocks produced from infected cell supernatant and, with further optimization, quantification of SARS-CoV-2 in specimens collected from infected animals. (c) 2019 The Authors. Basic Protocol: SARS-CoV-2 plaque assay using a solid double overlay method Alternate Protocol: SARS-CoV-2 plaque assay using a liquid overlay and fixation-staining method. Twit Text FOAVip Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS-CoV-2 ????Curr Protoc Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=32475066 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32475066 #FOAvip English Wikipedia <ref>{{Cite pmid|32475066}}</ref> Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS-CoV-2 #MMPMID32475066 Mendoza EJ; Manguiat K; Wood H; Drebot M Curr Protoc Microbiol 2020[Jun]; 57 (1): ecpmc105 PMID32475066show ga Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been identified as the causal agent of COronaVIrus Disease-19 (COVID-19), an atypical pneumonia-like syndrome that emerged in December 2019. While SARS-CoV-2 titers can be measured by detection of viral nucleic acid, this method is unable to quantitate infectious virions. Measurement of infectious SARS-CoV-2 can be achieved by tissue culture infectious dose-50 (TCID(50) ), which detects the presence or absence of cytopathic effect in cells infected with serial dilutions of a virus specimen. However, this method only provides a qualitative infectious virus titer. Plaque assays are a quantitative method of measuring infectious SARS-CoV-2 by quantifying the plaques formed in cell culture upon infection with serial dilutions of a virus specimen. As such, plaque assays remain the gold standard in quantifying concentrations of replication-competent lytic virions. Here, we describe two detailed plaque assay protocols to quantify infectious SARS-CoV-2 using different overlay and staining methods. Both methods have several advantages and disadvantages, which can be considered when choosing the procedure best suited for each laboratory. These assays can be used for several research purposes, including titration of virus stocks produced from infected cell supernatant and, with further optimization, quantification of SARS-CoV-2 in specimens collected from infected animals. (c) 2019 The Authors. Basic Protocol: SARS-CoV-2 plaque assay using a solid double overlay method Alternate Protocol: SARS-CoV-2 plaque assay using a liquid overlay and fixation-staining method. |*Clinical Protocols[MESH] |Animals[MESH] |Betacoronavirus/*isolation & purification[MESH] |Chlorocebus aethiops[MESH] |Humans[MESH] |SARS-CoV-2[MESH] |Staining and Labeling[MESH] |Vero Cells[MESH]Two Detailed Plaque Assay Protocols for the Quantification of Infectio(epmc).pdf DeepDyve Pubget Overpricing