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10.1016/j.neuint.2020.104779 http://scihub22266oqcxt.onion/10.1016/j.neuint.2020.104779 32474177!7256623!32474177     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Neurochem+Int 2020 ; 138 (ä): 104779 Nephropedia Template TP {{tp|p=32474177|t=2020. Nicotinic acetylcholine receptors regulate clustering, fusion and acidification of the rat brain synaptic vesicles |pdf=|usr=}}{{32474177}} gab.com Text Nicotinic acetylcholine receptors regulate clustering, fusion and acidification of the rat brain synaptic vesicles JO: Neurochem Int http://www.kidney.de/pget.php?&tw=1&pmid=32474177 #FOAvip The brain nicotinic acetylcholine receptors (nAChRs) expressed in pre-synaptic nerve terminals regulate neurotransmitter release. However, there is no evidence for the expression of nAChRs in synaptic vesicles, which deliver neurotransmitter to synaptic cleft. The aim of this paper was to investigate the presence of nAChRs in synaptic vesicles purified from the rat brain and to study their possible involvement in vesicles life cycle. According to dynamic light scattering analysis, the antibody against extracellular domain (1-208) of alpha7 nAChR subunit inhibited synaptic vesicles clustering. Sandwich ELISA with nAChR subunit-specific antibodies demonstrated the presence of alpha4beta2, alpha7 and alpha7beta2nAChR subtypes in synaptic vesicles and showed that alpha7 and beta2 nAChR subunits are co-localized with synaptic vesicle glycoprotein 2A (SV2A). Pre-incubation with either alpha7-selective agonist PNU282987 or nicotine did not affect synaptic vesicles clustering but delayed their Ca(2+)-dependent fusion with the plasma membranes. In contrast, nicotine but not PNU282987 stimulated acidification of isolated synaptic vesicles, indicating that alpha4beta2 but not alpha7-containing nAChRs are involved in regulation of proton influx and neurotransmitter refilling. Treatment of rats with levetiracetam, a specific modulator of SV2A, increased the content of alpha7 nAChRs in synaptic vesicles accompanied by increased clustering but decreased Ca(2+)-dependent fusion. These data for the first time demonstrate the presence of nAChRs in synaptic vesicles and suggest an active involvement of cholinergic regulation in neurotransmitter release. Synaptic vesicles may be an additional target of nicotine inhaled upon smoking and of alpha7-specific drugs widely discussed as anti-inflammatory and pro-cognitive tools. Twit Text FOAVip Nicotinic acetylcholine receptors regulate clustering, fusion and acidification of the rat brain synaptic vesicles ????Neurochem Int http://www.kidney.de/pget.php?&tw=1&pmid=32474177 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32474177 #FOAvip English Wikipedia <ref>{{Cite pmid|32474177}}</ref> Nicotinic acetylcholine receptors regulate clustering, fusion and acidification of the rat brain synaptic vesicles #MMPMID32474177 Trikash I; Kasatkina L; Lykhmus O; Skok M Neurochem Int 2020[Sep]; 138 (ä): 104779 PMID32474177show ga The brain nicotinic acetylcholine receptors (nAChRs) expressed in pre-synaptic nerve terminals regulate neurotransmitter release. However, there is no evidence for the expression of nAChRs in synaptic vesicles, which deliver neurotransmitter to synaptic cleft. The aim of this paper was to investigate the presence of nAChRs in synaptic vesicles purified from the rat brain and to study their possible involvement in vesicles life cycle. According to dynamic light scattering analysis, the antibody against extracellular domain (1-208) of alpha7 nAChR subunit inhibited synaptic vesicles clustering. Sandwich ELISA with nAChR subunit-specific antibodies demonstrated the presence of alpha4beta2, alpha7 and alpha7beta2nAChR subtypes in synaptic vesicles and showed that alpha7 and beta2 nAChR subunits are co-localized with synaptic vesicle glycoprotein 2A (SV2A). Pre-incubation with either alpha7-selective agonist PNU282987 or nicotine did not affect synaptic vesicles clustering but delayed their Ca(2+)-dependent fusion with the plasma membranes. In contrast, nicotine but not PNU282987 stimulated acidification of isolated synaptic vesicles, indicating that alpha4beta2 but not alpha7-containing nAChRs are involved in regulation of proton influx and neurotransmitter refilling. Treatment of rats with levetiracetam, a specific modulator of SV2A, increased the content of alpha7 nAChRs in synaptic vesicles accompanied by increased clustering but decreased Ca(2+)-dependent fusion. These data for the first time demonstrate the presence of nAChRs in synaptic vesicles and suggest an active involvement of cholinergic regulation in neurotransmitter release. Synaptic vesicles may be an additional target of nicotine inhaled upon smoking and of alpha7-specific drugs widely discussed as anti-inflammatory and pro-cognitive tools. |Animals[MESH] |Brain/drug effects/*metabolism[MESH] |Cell Membrane/drug effects/*metabolism[MESH] |Female[MESH] |Hydrogen-Ion Concentration[MESH] |Male[MESH] |Membrane Fusion/drug effects/*physiology[MESH] |Mice[MESH] |Mice, Inbred C57BL[MESH] |Mice, Knockout[MESH] |Nicotinic Agonists/pharmacology[MESH] |Nicotinic Antagonists/pharmacology[MESH] |Rats[MESH] |Rats, Wistar[MESH] |Synaptic Vesicles/drug effects/*metabolism[MESH]Nicotinic acetylcholine receptors regulate clustering, fusion and acid(epmc).pdf DeepDyve Pubget Overpricing