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10.1080/09273948.2020.1770301

http://scihub22266oqcxt.onion/10.1080/09273948.2020.1770301
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32469258!ä!32469258

suck abstract from ncbi


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pmid32469258      Ocul+Immunol+Inflamm 2020 ; 28 (5): 721-725
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  • Detection of SARS-CoV-2 in Human Retinal Biopsies of Deceased COVID-19 Patients #MMPMID32469258
  • Casagrande M; Fitzek A; Puschel K; Aleshcheva G; Schultheiss HP; Berneking L; Spitzer MS; Schultheiss M
  • Ocul Immunol Inflamm 2020[Jul]; 28 (5): 721-725 PMID32469258show ga
  • PURPOSE: To report the presence of viral ribonucleic acid (RNA) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human retina in deceased patients with confirmed novel coronavirus disease 2019 (COVID-19). PATIENTS AND METHODS: Fourteen eyes of 14 deceased patients with confirmed COVID-19 disease were enucleated during autopsy. A sample of human retina was secured and fixed in RNAlater. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to detect three different viral RNA sequences (RdRp-gene, E-gene and Orf1 gene) of SARS-CoV-2. RESULTS: In three out of 14 eyes SARS-CoV-2 viral RNA was detected in the retina of deceased COVID-19 patients. As analysis for three different sequences (RdRp-gene, E-gene and Orf1 gene) revealed positive results in RT-PCR, the existence of SARS-CoV-2 viral RNA in human retina is proven according to the standards of the World-Health-Organization. CONCLUSION: Viral RNA of SARS-CoV-2 is detectable in the retina of COVID-19 patients.
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |Autopsy[MESH]
  • |Betacoronavirus/genetics/*isolation & purification[MESH]
  • |Biopsy[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |COVID-19 Vaccines[MESH]
  • |Clinical Laboratory Techniques[MESH]
  • |Coronavirus Infections/diagnosis/mortality/*virology[MESH]
  • |Eye Enucleation[MESH]
  • |Eye Infections, Viral/mortality/*virology[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/mortality/*virology[MESH]
  • |Prospective Studies[MESH]
  • |RNA, Viral/genetics[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]
  • |Retinal Diseases/mortality/*virology[MESH]


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