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10.3390/v12060582 http://scihub22266oqcxt.onion/10.3390/v12060582 32466458!7354519!32466458     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32466458&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Viruses 2020 ; 12 (6): ? Nephropedia Template TP {{tp|p=32466458|t=2020. Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2 |pdf=|usr=}}{{32466458}} gab.com Text Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2 JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32466458 #FOAvip The recent outbreak of the Coronavirus disease 2019 (COVID-19) has quickly spread worldwide since its discovery in Wuhan city, China in December 2019. A comprehensive strategy, including surveillance, diagnostics, research, clinical treatment, and development of vaccines, is urgently needed to win the battle against COVID-19. The past three unprecedented outbreaks of emerging human coronavirus infections at the beginning of the 21st century have highlighted the importance of readily available, accurate, and rapid diagnostic technologies to contain emerging and re-emerging pandemics. Real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) based assays performed on respiratory specimens remain the gold standard for COVID-19 diagnostics. However, point-of-care technologies and serologic immunoassays are rapidly emerging with high sensitivity and specificity as well. Even though excellent techniques are available for the diagnosis of symptomatic patients with COVID-19 in well-equipped laboratories; critical gaps still remain in screening asymptomatic people who are in the incubation phase of the virus, as well as in the accurate determination of live viral shedding during convalescence to inform decisions for ending isolation. This review article aims to discuss the currently available laboratory methods and surveillance technologies available for the detection of COVID-19, their performance characteristics and highlight the gaps in current diagnostic capacity, and finally, propose potential solutions. We also summarize the specifications of the majority of the available commercial kits (PCR, EIA, and POC) for laboratory diagnosis of COVID-19. Twit Text FOAVip Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2 ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32466458 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32466458 #FOAvip English Wikipedia <ref>{{Cite pmid|32466458}}</ref> Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2 #MMPMID32466458 Younes N; Al-Sadeq DW; Al-Jighefee H; Younes S; Al-Jamal O; Daas HI; Yassine HM; Nasrallah GK Viruses 2020[May]; 12 (6): ? PMID32466458show ga The recent outbreak of the Coronavirus disease 2019 (COVID-19) has quickly spread worldwide since its discovery in Wuhan city, China in December 2019. A comprehensive strategy, including surveillance, diagnostics, research, clinical treatment, and development of vaccines, is urgently needed to win the battle against COVID-19. The past three unprecedented outbreaks of emerging human coronavirus infections at the beginning of the 21st century have highlighted the importance of readily available, accurate, and rapid diagnostic technologies to contain emerging and re-emerging pandemics. Real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) based assays performed on respiratory specimens remain the gold standard for COVID-19 diagnostics. However, point-of-care technologies and serologic immunoassays are rapidly emerging with high sensitivity and specificity as well. Even though excellent techniques are available for the diagnosis of symptomatic patients with COVID-19 in well-equipped laboratories; critical gaps still remain in screening asymptomatic people who are in the incubation phase of the virus, as well as in the accurate determination of live viral shedding during convalescence to inform decisions for ending isolation. This review article aims to discuss the currently available laboratory methods and surveillance technologies available for the detection of COVID-19, their performance characteristics and highlight the gaps in current diagnostic capacity, and finally, propose potential solutions. We also summarize the specifications of the majority of the available commercial kits (PCR, EIA, and POC) for laboratory diagnosis of COVID-19. |*Clinical Laboratory Techniques[MESH] |Asymptomatic Infections[MESH] |Betacoronavirus[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |COVID-19 Vaccines[MESH] |Coronavirus Infections/*diagnosis[MESH] |Humans[MESH] |Immunoenzyme Techniques[MESH] |Neutralization Tests[MESH] |Nucleic Acid Amplification Techniques[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis[MESH] |Point-of-Care Testing[MESH] |Reagent Kits, Diagnostic/standards[MESH] |Real-Time Polymerase Chain Reaction[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH] |Serologic Tests[MESH] |Tomography, X-Ray Computed[MESH]Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2(epmc).pdf DeepDyve Pubget Overpricing