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10.1016/j.scitotenv.2020.139652 http://scihub22266oqcxt.onion/10.1016/j.scitotenv.2020.139652 32464333!7245320!32464333     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Total+Environ 2020 ; 736 (ä): 139652 Nephropedia Template TP {{tp|p=32464333|t=2020. First detection of SARS-CoV-2 in untreated wastewaters in Italy |pdf=|usr=}}{{32464333}} gab.com Text First detection of SARS-CoV-2 in untreated wastewaters in Italy JO: Sci Total Environ http://www.kidney.de/pget.php?&tw=1&pmid=32464333 #FOAvip Several studies have demonstrated the advantages of environmental surveillance through the monitoring of sewage for the assessment of viruses circulating in a given community (wastewater-based epidemiology, WBE). During the COVID-19 public health emergency, many reports have described the presence of SARS-CoV-2 RNA in stools from COVID-19 patients, and a few studies reported the occurrence of SARS-CoV-2 in wastewaters worldwide. Italy is among the world's worst-affected countries in the COVID-19 pandemic, but so far there are no studies assessing the presence of SARS-CoV-2 in Italian wastewaters. To this aim, twelve influent sewage samples, collected between February and April 2020 from Wastewater Treatment Plants in Milan and Rome, were tested adapting, for concentration, the standard WHO procedure for Poliovirus surveillance. Molecular analysis was undertaken with three nested protocols, including a newly designed SARS-CoV-2 specific primer set. SARS-CoV-2 RNA detection was accomplished in volumes of 250 ml of wastewaters collected in areas of high (Milan) and low (Rome) epidemic circulation, according to clinical data. Overall, 6 out of 12 samples were positive. One of the positive results was obtained in a Milan wastewater sample collected a few days after the first notified Italian case of autochthonous SARS-CoV-2. The study confirms that WBE has the potential to be applied to SARS-CoV-2 as a sensitive tool to study spatial and temporal trends of virus circulation in the population. Twit Text FOAVip First detection of SARS-CoV-2 in untreated wastewaters in Italy ????Sci Total Environ http://www.kidney.de/pget.php?&tw=1&pmid=32464333 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32464333 #FOAvip English Wikipedia <ref>{{Cite pmid|32464333}}</ref> First detection of SARS-CoV-2 in untreated wastewaters in Italy #MMPMID32464333 La Rosa G; Iaconelli M; Mancini P; Bonanno Ferraro G; Veneri C; Bonadonna L; Lucentini L; Suffredini E Sci Total Environ 2020[Sep]; 736 (ä): 139652 PMID32464333show ga Several studies have demonstrated the advantages of environmental surveillance through the monitoring of sewage for the assessment of viruses circulating in a given community (wastewater-based epidemiology, WBE). During the COVID-19 public health emergency, many reports have described the presence of SARS-CoV-2 RNA in stools from COVID-19 patients, and a few studies reported the occurrence of SARS-CoV-2 in wastewaters worldwide. Italy is among the world's worst-affected countries in the COVID-19 pandemic, but so far there are no studies assessing the presence of SARS-CoV-2 in Italian wastewaters. To this aim, twelve influent sewage samples, collected between February and April 2020 from Wastewater Treatment Plants in Milan and Rome, were tested adapting, for concentration, the standard WHO procedure for Poliovirus surveillance. Molecular analysis was undertaken with three nested protocols, including a newly designed SARS-CoV-2 specific primer set. SARS-CoV-2 RNA detection was accomplished in volumes of 250 ml of wastewaters collected in areas of high (Milan) and low (Rome) epidemic circulation, according to clinical data. Overall, 6 out of 12 samples were positive. One of the positive results was obtained in a Milan wastewater sample collected a few days after the first notified Italian case of autochthonous SARS-CoV-2. The study confirms that WBE has the potential to be applied to SARS-CoV-2 as a sensitive tool to study spatial and temporal trends of virus circulation in the population. |*Coronavirus Infections[MESH] |*Pandemics[MESH] |*Pneumonia, Viral[MESH] |Betacoronavirus/*isolation & purification[MESH] |COVID-19[MESH] |Humans[MESH] |Italy[MESH] |RNA, Viral/isolation & purification[MESH] |Real-Time Polymerase Chain Reaction[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |SARS-CoV-2[MESH] |Sewage/virology[MESH]First detection of SARS-CoV-2 in untreated wastewaters in Italy (epmc).pdf DeepDyve Pubget Overpricing