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10.1007/s10096-020-03930-8 http://scihub22266oqcxt.onion/10.1007/s10096-020-03930-8 32462501!8824685!32462501     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32462501&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Eur+J+Clin+Microbiol+Infect+Dis 2020 ; 39 (10): 1997-2000 Nephropedia Template TP {{tp|p=32462501|t=2020. The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?|pdf=|usr=}}{{32462501}} gab.com Text The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis JO: Eur J Clin Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32462501 #FOAvip Several commercial assays for SARS-CoV-2 RT-PCR are available but few of them were assessed. We evaluate the Allplex 2019-nCoV (Seegene) assay using 41 nasopharyngeal samples. The rates of agreement were 92.7% and 100% with the GeneFinder COVID-19 plus (Elitech) and the diagnosis of the infectious disease specialist respectively. Four samples display a Ct < 22.0 for the E and RdRp genes while the N gene was not detected, suggesting a variability of the viral sequence. There was no cross-reactivity with other respiratory viruses. The Allplex 2019-nCoV appears as a reliable method, but additional evaluations using more samples are needed. RT-PCR assays should probably include at least 2 viral targets. Twit Text FOAVip The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis ????Eur J Clin Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32462501 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32462501 #FOAvip English Wikipedia <ref>{{Cite pmid|32462501}}</ref> The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis? #MMPMID32462501 Farfour E; Lesprit P; Visseaux B; Pascreau T; Jolly E; Houhou N; Mazaux L; Asso-Bonnet M; Vasse M Eur J Clin Microbiol Infect Dis 2020[Oct]; 39 (10): 1997-2000 PMID32462501show ga Several commercial assays for SARS-CoV-2 RT-PCR are available but few of them were assessed. We evaluate the Allplex 2019-nCoV (Seegene) assay using 41 nasopharyngeal samples. The rates of agreement were 92.7% and 100% with the GeneFinder COVID-19 plus (Elitech) and the diagnosis of the infectious disease specialist respectively. Four samples display a Ct < 22.0 for the E and RdRp genes while the N gene was not detected, suggesting a variability of the viral sequence. There was no cross-reactivity with other respiratory viruses. The Allplex 2019-nCoV appears as a reliable method, but additional evaluations using more samples are needed. RT-PCR assays should probably include at least 2 viral targets. |Betacoronavirus/*genetics[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/methods[MESH] |Coronavirus Envelope Proteins[MESH] |Coronavirus Infections/*diagnosis[MESH] |Coronavirus Nucleocapsid Proteins[MESH] |Coronavirus RNA-Dependent RNA Polymerase[MESH] |France[MESH] |Humans[MESH] |Nasopharynx/virology[MESH] |Nucleocapsid Proteins/*genetics[MESH] |Pandemics[MESH] |Phosphoproteins[MESH] |Pneumonia, Viral/*diagnosis[MESH] |Prospective Studies[MESH] |RNA-Dependent RNA Polymerase/*genetics[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2[MESH] |Viral Envelope Proteins/*genetics[MESH]The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS(epmc).pdf DeepDyve Pubget Overpricing