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10.1016/j.jcv.2020.104448

http://scihub22266oqcxt.onion/10.1016/j.jcv.2020.104448
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32460173!7236671!32460173
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suck abstract from ncbi


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pmid32460173      J+Clin+Virol 2020 ; 128 (ä): 104448
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  • Clinical evaluation of AusDiagnostics SARS-CoV-2 multiplex tandem PCR assay #MMPMID32460173
  • Attwood LO; Francis MJ; Hamblin J; Korman TM; Druce J; Graham M
  • J Clin Virol 2020[Jul]; 128 (ä): 104448 PMID32460173show ga
  • BACKGROUND: In the context of the pandemic, the rapid emergency use authorisation of diagnostic assays for SARS-CoV-2 has meant there are few peer-reviewed published studies of clinical performance of commercial assays. AIMS: To evaluate the clinical performance of AusDiagnostics respiratory multiplex tandem PCR assay including SARS-CoV-2. METHODS: We reviewed the results following implementation of AusDiagnostics respiratory multiplex tandem PCR assay including SARS-CoV-2, and compared with an in-house RT-PCR assay at our State Reference Laboratory. RESULTS: Initial validation using AusDiagnostics coronavirus multiplex tandem PCR assay including SARS-CoV-2 demonstrated good concordance with the State Reference Laboratory. After implementing the AusDiagnostics respiratory multiplex tandem PCR assay including SARS-CoV-2, we tested 7839 samples. 127 samples in which SARS-CoV-2 was detected using the AusDiagnostics assay were referred for testing at the State Reference Laboratory, with concordant results in 118/127 (92.9%) of samples. After resolution of discrepancies, 125/127 (98.4%) of AusDiagnostics results were determined to be true positive results. Out of 7839 samples tested for SARS-CoV-2 during this period, only 2 tests (0.02%) were indeterminate results. CONCLUSION: The AusDiagnostics respiratory MT-PCR assay is a reliable assay for detection of SARS-CoV-2.
  • |*Pandemics[MESH]
  • |Adult[MESH]
  • |Betacoronavirus/genetics/*isolation & purification[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Clinical Laboratory Techniques/*methods[MESH]
  • |Coronavirus Infections/*diagnosis/epidemiology/virology[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Multiplex Polymerase Chain Reaction/*methods[MESH]
  • |Nasopharynx/virology[MESH]
  • |Oropharynx/virology[MESH]
  • |Pneumonia, Viral/*diagnosis/epidemiology/virology[MESH]
  • |Reproducibility of Results[MESH]
  • |SARS-CoV-2[MESH]


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