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10.1021/acs.analchem.0c01032 http://scihub22266oqcxt.onion/10.1021/acs.analchem.0c01032 32441935!ä!32441935 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Chem 2020 ; 92 (14): 9699-9705 Nephropedia Template TP {{tp|p=32441935|t=2020. Reverse-Transcription Recombinase-Aided Amplification Assay for Rapid Detection of the 2019 Novel Coronavirus (SARS-CoV-2) |pdf=|usr=}}{{32441935}} gab.com Text Reverse-Transcription Recombinase-Aided Amplification Assay for Rapid Detection of the 2019 Novel Coronavirus (SARS-CoV-2) JO: Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=32441935 #FOAvip A novel coronavirus (SARS-CoV-2) was recently identified in patients with acute respiratory disease and spread quickly worldwide. A specific and rapid diagnostic method is important for early identification. The reverse-transcription recombinase-aided amplification (RT-RAA) assay is a rapid detection method for several pathogens. Assays were performed within 5-15 min as a one-step single tube reaction at 39 degrees C. In this study, we established two RT-RAA assays for the S and orf1ab gene of SARS-CoV-2 using clinical specimens for validation. The analytical sensitivity of the RT-RAA assay was 10 copies for the S and one copy for the orf1ab gene per reaction. Cross-reactions were not observed with any of the other respiratory pathogens. A 100% agreement between the RT-RAA and real-time PCR assays was accomplished after testing 120 respiratory specimens. These results demonstrate that the proposed RT-RAA assay will be beneficial as it is a faster, more sensitive, and more specific tool for the detection of SARS-CoV-2. Twit Text FOAVip Reverse-Transcription Recombinase-Aided Amplification Assay for Rapid Detection of the 2019 Novel Coronavirus (SARS-CoV-2) ????Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=32441935 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32441935 #FOAvip English Wikipedia <ref>{{Cite pmid|32441935}}</ref> Reverse-Transcription Recombinase-Aided Amplification Assay for Rapid Detection of the 2019 Novel Coronavirus (SARS-CoV-2) #MMPMID32441935 Xue G; Li S; Zhang W; Du B; Cui J; Yan C; Huang L; Chen L; Zhao L; Sun Y; Li N; Zhao H; Feng Y; Wang Z; Liu S; Zhang Q; Xie X; Liu D; Yao H; Yuan J Anal Chem 2020[Jul]; 92 (14): 9699-9705 PMID32441935show ga A novel coronavirus (SARS-CoV-2) was recently identified in patients with acute respiratory disease and spread quickly worldwide. A specific and rapid diagnostic method is important for early identification. The reverse-transcription recombinase-aided amplification (RT-RAA) assay is a rapid detection method for several pathogens. Assays were performed within 5-15 min as a one-step single tube reaction at 39 degrees C. In this study, we established two RT-RAA assays for the S and orf1ab gene of SARS-CoV-2 using clinical specimens for validation. The analytical sensitivity of the RT-RAA assay was 10 copies for the S and one copy for the orf1ab gene per reaction. Cross-reactions were not observed with any of the other respiratory pathogens. A 100% agreement between the RT-RAA and real-time PCR assays was accomplished after testing 120 respiratory specimens. These results demonstrate that the proposed RT-RAA assay will be beneficial as it is a faster, more sensitive, and more specific tool for the detection of SARS-CoV-2. |Bacteria/chemistry/genetics[MESH] |Betacoronavirus/*chemistry/*genetics[MESH] |COVID-19[MESH] |Coronavirus Infections/*diagnosis[MESH] |Cross Reactions[MESH] |DNA Probes[MESH] |Genes, Viral[MESH] |Humans[MESH] |Nucleic Acid Amplification Techniques/*methods[MESH] |Pandemics[MESH] |Plasmids[MESH] |Pneumonia, Viral/*diagnosis[MESH] |Polymerase Chain Reaction/*methods[MESH] |Polyproteins[MESH] |Respiratory Tract Infections/microbiology/virology[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH] |Viral Proteins/genetics[MESH]Reverse-Transcription Recombinase-Aided Amplification Assay for Rapid (epmc).pdf DeepDyve Pubget Overpricing