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10.1093/rb/rbz033 http://scihub22266oqcxt.onion/10.1093/rb/rbz033 32440359!7233620!32440359     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32440359&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Regen+Biomater 2020 ; 7 (1): 53-61 Nephropedia Template TP {{tp|p=32440359|t=2020. Effect of magnesium ions/Type I collagen promote the biological behavior of osteoblasts and its mechanism |pdf=|usr=}}{{32440359}} gab.com Text Effect of magnesium ions/Type I collagen promote the biological behavior of osteoblasts and its mechanism JO: Regen Biomater http://www.kidney.de/pget.php?&tw=1&pmid=32440359 #FOAvip Type I collagen (Col I) is a main component of extracellular matrix (ECM). Its safety, biocompatibility, hydrophilicity and pyrogen immunogenicity make it suitable for tissues engineering applications. Mg(2+) also control a myriad of cellular processes, including the bone development by enhancing the attachment and differentiation of osteoblasts and accelerating mineralization to enhance bone healing. In our studies, Mg(2+) bind collagen to promote the proliferation and differentiation of osteoblasts through the expression of integrins and downstream signaling pathways. In order to clarify the biological behavior effect of 10 mM Mg(2+)/Col I coating, we performed 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline phosphatase (ALP), 4'6-diamidino-2-phenylindole (DAPI), Alizarin red staining and Rhodamine B-isothiocyanate (RITC)-labeled phalloidin experiments and found that 10 mM Mg(2+) group, Col I-coating group, 10 mM Mg(2+)/Col I-coating group, respectively, promoted the proliferation and differentiation of osteoblasts, especially 10 mM Mg(2+)/Col I-coating group. We detected the mRNA expression of osteogenic-related genes (Runx2, ALP and OCN, OPN and BMP-2) and the protein expression of signaling pathway (integrin alpha2, integrin beta1, FAK and ERK1/2), these results indicated that 10 mM Mg(2+)/Col I coating play an critical role in up-regulating the MC3T3-E1 cells activity. The potential mechanisms of this specific performance may be through activating via integrin alpha2beta1-FAK-ERK1/2 protein-coupled receptor pathway. Twit Text FOAVip Effect of magnesium ions/Type I collagen promote the biological behavior of osteoblasts and its mechanism ????Regen Biomater http://www.kidney.de/pget.php?&tw=1&pmid=32440359 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32440359 #FOAvip English Wikipedia <ref>{{Cite pmid|32440359}}</ref> Effect of magnesium ions/Type I collagen promote the biological behavior of osteoblasts and its mechanism #MMPMID32440359 Nie X; Sun X; Wang C; Yang J Regen Biomater 2020[Feb]; 7 (1): 53-61 PMID32440359show ga Type I collagen (Col I) is a main component of extracellular matrix (ECM). Its safety, biocompatibility, hydrophilicity and pyrogen immunogenicity make it suitable for tissues engineering applications. Mg(2+) also control a myriad of cellular processes, including the bone development by enhancing the attachment and differentiation of osteoblasts and accelerating mineralization to enhance bone healing. In our studies, Mg(2+) bind collagen to promote the proliferation and differentiation of osteoblasts through the expression of integrins and downstream signaling pathways. In order to clarify the biological behavior effect of 10 mM Mg(2+)/Col I coating, we performed 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline phosphatase (ALP), 4'6-diamidino-2-phenylindole (DAPI), Alizarin red staining and Rhodamine B-isothiocyanate (RITC)-labeled phalloidin experiments and found that 10 mM Mg(2+) group, Col I-coating group, 10 mM Mg(2+)/Col I-coating group, respectively, promoted the proliferation and differentiation of osteoblasts, especially 10 mM Mg(2+)/Col I-coating group. We detected the mRNA expression of osteogenic-related genes (Runx2, ALP and OCN, OPN and BMP-2) and the protein expression of signaling pathway (integrin alpha2, integrin beta1, FAK and ERK1/2), these results indicated that 10 mM Mg(2+)/Col I coating play an critical role in up-regulating the MC3T3-E1 cells activity. The potential mechanisms of this specific performance may be through activating via integrin alpha2beta1-FAK-ERK1/2 protein-coupled receptor pathway. ?Effect of magnesium ions/Type I collagen promote the biological behavi(epmc).pdf DeepDyve Pubget Overpricing