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10.1164/rccm.202003-0541OC http://scihub22266oqcxt.onion/10.1164/rccm.202003-0541OC 32432483!7365377!32432483     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Respir+Crit+Care+Med 2020 ; 202 (2): 219-229 Nephropedia Template TP {{tp|p=32432483|t=2020. Expression of the SARS-CoV-2 ACE2 Receptor in the Human Airway Epithelium |pdf=|usr=}}{{32432483}} gab.com Text Expression of the SARS-CoV-2 ACE2 Receptor in the Human Airway Epithelium JO: Am J Respir Crit Care Med http://www.kidney.de/pget.php?&tw=1&pmid=32432483 #FOAvip Rationale: Infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease (COVID-19), a predominantly respiratory illness. The first step in SARS-CoV-2 infection is binding of the virus to ACE2 (angiotensin-converting enzyme 2) on the airway epithelium.Objectives: The objective was to gain insight into the expression of ACE2 in the human airway epithelium.Methods: Airway epithelia sampled by fiberoptic bronchoscopy of trachea, large airway epithelia (LAE), and small airway epithelia (SAE) of nonsmokers and smokers were analyzed for expression of ACE2 and other coronavirus infection-related genes using microarray, RNA sequencing, and 10x single-cell transcriptome analysis, with associated examination of ACE2-related microRNA.Measurements and Main Results:1) ACE2 is expressed similarly in the trachea and LAE, with lower expression in the SAE; 2) in the SAE, ACE2 is expressed in basal, intermediate, club, mucus, and ciliated cells; 3) ACE2 is upregulated in the SAE by smoking, significantly in men; 4) levels of miR-1246 expression could play a role in ACE2 upregulation in the SAE of smokers; and 5) ACE2 is expressed in airway epithelium differentiated in vitro on air-liquid interface cultures from primary airway basal stem/progenitor cells; this can be replicated using LAE and SAE immortalized basal cell lines derived from healthy nonsmokers.Conclusions:ACE2, the gene encoding the receptor for SARS-CoV-2, is expressed in the human airway epithelium, with variations in expression relevant to the biology of initial steps in SARS-CoV-2 infection. Twit Text FOAVip Expression of the SARS-CoV-2 ACE2 Receptor in the Human Airway Epithelium ????Am J Respir Crit Care Med http://www.kidney.de/pget.php?&tw=1&pmid=32432483 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32432483 #FOAvip English Wikipedia <ref>{{Cite pmid|32432483}}</ref> Expression of the SARS-CoV-2 ACE2 Receptor in the Human Airway Epithelium #MMPMID32432483 Zhang H; Rostami MR; Leopold PL; Mezey JG; O'Beirne SL; Strulovici-Barel Y; Crystal RG Am J Respir Crit Care Med 2020[Jul]; 202 (2): 219-229 PMID32432483show ga Rationale: Infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease (COVID-19), a predominantly respiratory illness. The first step in SARS-CoV-2 infection is binding of the virus to ACE2 (angiotensin-converting enzyme 2) on the airway epithelium.Objectives: The objective was to gain insight into the expression of ACE2 in the human airway epithelium.Methods: Airway epithelia sampled by fiberoptic bronchoscopy of trachea, large airway epithelia (LAE), and small airway epithelia (SAE) of nonsmokers and smokers were analyzed for expression of ACE2 and other coronavirus infection-related genes using microarray, RNA sequencing, and 10x single-cell transcriptome analysis, with associated examination of ACE2-related microRNA.Measurements and Main Results:1) ACE2 is expressed similarly in the trachea and LAE, with lower expression in the SAE; 2) in the SAE, ACE2 is expressed in basal, intermediate, club, mucus, and ciliated cells; 3) ACE2 is upregulated in the SAE by smoking, significantly in men; 4) levels of miR-1246 expression could play a role in ACE2 upregulation in the SAE of smokers; and 5) ACE2 is expressed in airway epithelium differentiated in vitro on air-liquid interface cultures from primary airway basal stem/progenitor cells; this can be replicated using LAE and SAE immortalized basal cell lines derived from healthy nonsmokers.Conclusions:ACE2, the gene encoding the receptor for SARS-CoV-2, is expressed in the human airway epithelium, with variations in expression relevant to the biology of initial steps in SARS-CoV-2 infection. |*Betacoronavirus[MESH] |Angiotensin-Converting Enzyme 2[MESH] |COVID-19[MESH] |Case-Control Studies[MESH] |Coronavirus Infections/*metabolism[MESH] |Female[MESH] |Humans[MESH] |Lung/metabolism[MESH] |Male[MESH] |Pandemics[MESH] |Peptidyl-Dipeptidase A/*genetics/*metabolism[MESH] |Pneumonia, Viral/*metabolism[MESH] |RNA, Messenger/genetics/metabolism[MESH] |Respiratory Mucosa/*metabolism[MESH] |SARS-CoV-2[MESH] |Sex Factors[MESH] |Smoking/metabolism[MESH]Expression of the SARS-CoV-2 ACE2 Receptor in the Human Airway Epithel(epmc).pdf DeepDyve Pubget Overpricing