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10.1093/infdis/jiaa272

http://scihub22266oqcxt.onion/10.1093/infdis/jiaa272
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32427340!7313924!32427340
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suck abstract from ncbi


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pmid32427340      J+Infect+Dis 2020 ; 222 (2): 203-205
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  • Influence of Storage Conditions on SARS-CoV-2 Nucleic Acid Detection in Throat Swabs #MMPMID32427340
  • Li L; Li X; Guo Z; Wang Z; Zhang K; Li C; Wang C; Zhang S
  • J Infect Dis 2020[Jun]; 222 (2): 203-205 PMID32427340show ga
  • The detection of SARS-CoV-2 infection is the premise of quarantine. In many countries or areas, samples need to be shipped or inactivated before SARS-CoV-2 testing. In this study, we checked the influence of sample storage conditions on SARS-CoV-2 nucleic acid testing results, including sample inactivation time, storage temperature, and storage time. All of these conditions caused an increase in the cycle threshold values of the nucleic acid tests and led to the misclassification of at least 10.2% of positive cases as negative or suspected. The results highlight the importance of immediate testing of samples for SARS-CoV-2 nucleic acid detection.
  • |*Betacoronavirus/genetics[MESH]
  • |*Clinical Laboratory Techniques[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Coronavirus Infections/*diagnosis[MESH]
  • |Cryopreservation[MESH]
  • |Freezing[MESH]
  • |Humans[MESH]
  • |Pandemics[MESH]
  • |Pharynx/*virology[MESH]
  • |Pneumonia, Viral/*diagnosis[MESH]
  • |Refrigeration[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction[MESH]
  • |SARS-CoV-2[MESH]
  • |Specimen Handling/*methods[MESH]
  • |Temperature[MESH]
  • |Time Factors[MESH]


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