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10.7554/eLife.55799 http://scihub22266oqcxt.onion/10.7554/eLife.55799 32427100!7282821!32427100     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Elife 2020 ; 9 (ä): ä Nephropedia Template TP {{tp|p=32427100|t=2020. mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding |pdf=|usr=}}{{32427100}} gab.com Text mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding JO: Elife http://www.kidney.de/pget.php?&tw=1&pmid=32427100 #FOAvip Although the elongating ribosome is an efficient helicase, certain mRNA stem-loop structures are known to impede ribosome movement along mRNA and stimulate programmed ribosome frameshifting via mechanisms that are not well understood. Using biochemical and single-molecule Forster resonance energy transfer (smFRET) experiments, we studied how frameshift-inducing stem-loops from E. coli dnaX mRNA and the gag-pol transcript of Human Immunodeficiency Virus (HIV) perturb translation elongation. We find that upon encountering the ribosome, the stem-loops strongly inhibit A-site tRNA binding and ribosome intersubunit rotation that accompanies translation elongation. Electron cryo-microscopy (cryo-EM) reveals that the HIV stem-loop docks into the A site of the ribosome. Our results suggest that mRNA stem-loops can transiently escape the ribosome helicase by binding to the A site. Thus, the stem-loops can modulate gene expression by sterically hindering tRNA binding and inhibiting translation elongation. Twit Text FOAVip mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding ????Elife http://www.kidney.de/pget.php?&tw=1&pmid=32427100 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32427100 #FOAvip English Wikipedia <ref>{{Cite pmid|32427100}}</ref> mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding #MMPMID32427100 Bao C; Loerch S; Ling C; Korostelev AA; Grigorieff N; Ermolenko DN Elife 2020[May]; 9 (ä): ä PMID32427100show ga Although the elongating ribosome is an efficient helicase, certain mRNA stem-loop structures are known to impede ribosome movement along mRNA and stimulate programmed ribosome frameshifting via mechanisms that are not well understood. Using biochemical and single-molecule Forster resonance energy transfer (smFRET) experiments, we studied how frameshift-inducing stem-loops from E. coli dnaX mRNA and the gag-pol transcript of Human Immunodeficiency Virus (HIV) perturb translation elongation. We find that upon encountering the ribosome, the stem-loops strongly inhibit A-site tRNA binding and ribosome intersubunit rotation that accompanies translation elongation. Electron cryo-microscopy (cryo-EM) reveals that the HIV stem-loop docks into the A site of the ribosome. Our results suggest that mRNA stem-loops can transiently escape the ribosome helicase by binding to the A site. Thus, the stem-loops can modulate gene expression by sterically hindering tRNA binding and inhibiting translation elongation. |*Nucleic Acid Conformation[MESH] |*Ribosomes[MESH] |Bacterial Proteins/genetics/metabolism[MESH] |Cryoelectron Microscopy[MESH] |DNA Polymerase III/genetics/metabolism[MESH] |Escherichia coli/genetics/metabolism[MESH] |Frameshifting, Ribosomal[MESH] |Fusion Proteins, gag-pol[MESH] |Gene Expression Regulation, Bacterial[MESH] |Gene Expression Regulation, Viral[MESH] |HIV-1/genetics/metabolism[MESH] |RNA, Bacterial[MESH] |RNA, Messenger/chemistry/*metabolism[MESH]mRNA stem-loops can pause the ribosome by hindering A-site tRNA bindin(epmc).pdf DeepDyve Pubget Overpricing