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10.1016/j.mcp.2020.101599 http://scihub22266oqcxt.onion/10.1016/j.mcp.2020.101599 32425334!7233248!32425334     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32425334&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell+Probes 2020 ; 53 (ä): 101599 Nephropedia Template TP {{tp|p=32425334|t=2020. Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal |pdf=|usr=}}{{32425334}} gab.com Text Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal JO: Mol Cell Probes http://www.kidney.de/pget.php?&tw=1&pmid=32425334 #FOAvip *Most of the COVID-19 cases in Nepal are in the Southern districts of Nepal bordering India with travel histories to India.*Very few positive cases of COVID-19 are detected in Nepal which could either be due to early national lockdown.*Low PCR positivity rates could also be due to inefficiency of the PCR methods.*Whole genomes of 93 clinical samples from COVID-19 patients were analyzed to find the primer and probe binding sites.*Mutations in probe binding sites were found which could impact PCR efficiency resulting in false negative results. Twit Text FOAVip Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal ????Mol Cell Probes http://www.kidney.de/pget.php?&tw=1&pmid=32425334 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32425334 #FOAvip English Wikipedia <ref>{{Cite pmid|32425334}}</ref> Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal #MMPMID32425334 Rana DR; Pokhrel N Mol Cell Probes 2020[Oct]; 53 (ä): 101599 PMID32425334show ga *Most of the COVID-19 cases in Nepal are in the Southern districts of Nepal bordering India with travel histories to India.*Very few positive cases of COVID-19 are detected in Nepal which could either be due to early national lockdown.*Low PCR positivity rates could also be due to inefficiency of the PCR methods.*Whole genomes of 93 clinical samples from COVID-19 patients were analyzed to find the primer and probe binding sites.*Mutations in probe binding sites were found which could impact PCR efficiency resulting in false negative results. |*Genome, Viral[MESH] |*Pandemics[MESH] |Betacoronavirus/classification/*genetics/pathogenicity[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/methods[MESH] |Coronavirus Infections/*diagnosis/*epidemiology/transmission/virology[MESH] |DNA Probes/standards[MESH] |False Negative Reactions[MESH] |Humans[MESH] |India/epidemiology[MESH] |Mutation[MESH] |Nepal/epidemiology[MESH] |Pneumonia, Viral/*diagnosis/*epidemiology/transmission/virology[MESH] |RNA, Viral/*genetics[MESH] |Reagent Kits, Diagnostic/standards[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*standards[MESH] |SARS-CoV-2[MESH] |Severity of Illness Index[MESH]Sequence mismatch in PCR probes may mask the COVID-19 detection in Nep(epmc).pdf DeepDyve Pubget Overpricing