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Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Mol+Cell+Probes 2020 ; 53 (ä): 101599 Nephropedia Template TP
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Sequence mismatch in PCR probes may mask the COVID-19 detection in Nepal #MMPMID32425334
Rana DR; Pokhrel N
Mol Cell Probes 2020[Oct]; 53 (ä): 101599 PMID32425334show ga
*Most of the COVID-19 cases in Nepal are in the Southern districts of Nepal bordering India with travel histories to India.*Very few positive cases of COVID-19 are detected in Nepal which could either be due to early national lockdown.*Low PCR positivity rates could also be due to inefficiency of the PCR methods.*Whole genomes of 93 clinical samples from COVID-19 patients were analyzed to find the primer and probe binding sites.*Mutations in probe binding sites were found which could impact PCR efficiency resulting in false negative results.