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10.1016/j.cell.2020.05.025

http://scihub22266oqcxt.onion/10.1016/j.cell.2020.05.025
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32425270!7231725!32425270
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suck abstract from ncbi


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pmid32425270      Cell 2020 ; 182 (1): 73-84.e16
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  • Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients B Cells #MMPMID32425270
  • Cao Y; Su B; Guo X; Sun W; Deng Y; Bao L; Zhu Q; Zhang X; Zheng Y; Geng C; Chai X; He R; Li X; Lv Q; Zhu H; Deng W; Xu Y; Wang Y; Qiao L; Tan Y; Song L; Wang G; Du X; Gao N; Liu J; Xiao J; Su XD; Du Z; Feng Y; Qin C; Qin C; Jin R; Xie XS
  • Cell 2020[Jul]; 182 (1): 73-84.e16 PMID32425270show ga
  • The COVID-19 pandemic urgently needs therapeutic and prophylactic interventions. Here, we report the rapid identification of SARS-CoV-2-neutralizing antibodies by high-throughput single-cell RNA and VDJ sequencing of antigen-enriched B cells from 60 convalescent patients. From 8,558 antigen-binding IgG1(+) clonotypes, 14 potent neutralizing antibodies were identified, with the most potent one, BD-368-2, exhibiting an IC(50) of 1.2 and 15 ng/mL against pseudotyped and authentic SARS-CoV-2, respectively. BD-368-2 also displayed strong therapeutic and prophylactic efficacy in SARS-CoV-2-infected hACE2-transgenic mice. Additionally, the 3.8 A cryo-EM structure of a neutralizing antibody in complex with the spike-ectodomain trimer revealed the antibody's epitope overlaps with the ACE2 binding site. Moreover, we demonstrated that SARS-CoV-2-neutralizing antibodies could be directly selected based on similarities of their predicted CDR3(H) structures to those of SARS-CoV-neutralizing antibodies. Altogether, we showed that human neutralizing antibodies could be efficiently discovered by high-throughput single B cell sequencing in response to pandemic infectious diseases.
  • |*Single-Cell Analysis[MESH]
  • |Animals[MESH]
  • |Antibodies, Monoclonal/administration & dosage/chemistry/*isolation & purification/metabolism[MESH]
  • |Antibodies, Neutralizing/administration & dosage/chemistry/*isolation & purification/metabolism[MESH]
  • |B-Lymphocytes/*immunology[MESH]
  • |COVID-19[MESH]
  • |Convalescence[MESH]
  • |Coronavirus Infections/*immunology[MESH]
  • |High-Throughput Nucleotide Sequencing[MESH]
  • |Humans[MESH]
  • |Mice[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*immunology[MESH]
  • |Sequence Analysis, RNA[MESH]
  • |Spike Glycoprotein, Coronavirus/chemistry/metabolism[MESH]


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