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10.1016/j.watres.2020.115942 http://scihub22266oqcxt.onion/10.1016/j.watres.2020.115942 32425251!7229723!32425251     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Water+Res 2020 ; 181 (ä): 115942 Nephropedia Template TP {{tp|p=32425251|t=2020. SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area |pdf=|usr=}}{{32425251}} gab.com Text SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area JO: Water Res http://www.kidney.de/pget.php?&tw=1&pmid=32425251 #FOAvip Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused more than 200,000 reported COVID-19 cases in Spain resulting in more than 20,800 deaths as of April 21, 2020. Faecal shedding of SARS-CoV-2 RNA from COVID-19 patients has extensively been reported. Therefore, we investigated the occurrence of SARS-CoV-2 RNA in six wastewater treatments plants (WWTPs) serving the major municipalities within the Region of Murcia (Spain), the area with the lowest COVID-19 prevalence within Iberian Peninsula. Firstly, an aluminum hydroxide adsorption-precipitation concentration method was validated using a porcine coronavirus (Porcine Epidemic Diarrhea Virus, PEDV) and mengovirus (MgV). The procedure resulted in average recoveries of 10 +/- 3.5% and 10 +/- 2.1% in influent water (n = 2) and 3.3 +/- 1.6% and 6.2 +/- 1.0% in effluent water (n = 2) samples for PEDV and MgV, respectively. Then, the method was used to monitor the occurrence of SARS-CoV-2 from March 12 to April 14, 2020 in influent, secondary and tertiary effluent water samples. By using the real-time RT-PCR (RT-qPCR) Diagnostic Panel validated by US CDC that targets three regions of the virus nucleocapsid (N) gene, we estimated quantification of SARS-CoV-2 RNA titers in untreated wastewater samples of 5.4 +/- 0.2 log(10) genomic copies/L on average. Two secondary water samples resulted positive (2 out of 18) and all tertiary water samples tested as negative (0 out 12). This environmental surveillance data were compared to declared COVID-19 cases at municipality level, revealing that members of the community were shedding SARS-CoV-2 RNA in their stool even before the first cases were reported by local or national authorities in many of the cities where wastewaters have been sampled. The detection of SARS-CoV-2 in wastewater in early stages of the spread of COVID-19 highlights the relevance of this strategy as an early indicator of the infection within a specific population. At this point, this environmental surveillance could be implemented by municipalities right away as a tool, designed to help authorities to coordinate the exit strategy to gradually lift its coronavirus lockdown. Twit Text FOAVip SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area ????Water Res http://www.kidney.de/pget.php?&tw=1&pmid=32425251 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32425251 #FOAvip English Wikipedia <ref>{{Cite pmid|32425251}}</ref> SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area #MMPMID32425251 Randazzo W; Truchado P; Cuevas-Ferrando E; Simon P; Allende A; Sanchez G Water Res 2020[Aug]; 181 (ä): 115942 PMID32425251show ga Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused more than 200,000 reported COVID-19 cases in Spain resulting in more than 20,800 deaths as of April 21, 2020. Faecal shedding of SARS-CoV-2 RNA from COVID-19 patients has extensively been reported. Therefore, we investigated the occurrence of SARS-CoV-2 RNA in six wastewater treatments plants (WWTPs) serving the major municipalities within the Region of Murcia (Spain), the area with the lowest COVID-19 prevalence within Iberian Peninsula. Firstly, an aluminum hydroxide adsorption-precipitation concentration method was validated using a porcine coronavirus (Porcine Epidemic Diarrhea Virus, PEDV) and mengovirus (MgV). The procedure resulted in average recoveries of 10 +/- 3.5% and 10 +/- 2.1% in influent water (n = 2) and 3.3 +/- 1.6% and 6.2 +/- 1.0% in effluent water (n = 2) samples for PEDV and MgV, respectively. Then, the method was used to monitor the occurrence of SARS-CoV-2 from March 12 to April 14, 2020 in influent, secondary and tertiary effluent water samples. By using the real-time RT-PCR (RT-qPCR) Diagnostic Panel validated by US CDC that targets three regions of the virus nucleocapsid (N) gene, we estimated quantification of SARS-CoV-2 RNA titers in untreated wastewater samples of 5.4 +/- 0.2 log(10) genomic copies/L on average. Two secondary water samples resulted positive (2 out of 18) and all tertiary water samples tested as negative (0 out 12). This environmental surveillance data were compared to declared COVID-19 cases at municipality level, revealing that members of the community were shedding SARS-CoV-2 RNA in their stool even before the first cases were reported by local or national authorities in many of the cities where wastewaters have been sampled. The detection of SARS-CoV-2 in wastewater in early stages of the spread of COVID-19 highlights the relevance of this strategy as an early indicator of the infection within a specific population. At this point, this environmental surveillance could be implemented by municipalities right away as a tool, designed to help authorities to coordinate the exit strategy to gradually lift its coronavirus lockdown. |*Severe acute respiratory syndrome-related coronavirus[MESH] |Animals[MESH] |Betacoronavirus[MESH] |COVID-19[MESH] |Coronavirus Infections[MESH] |Humans[MESH] |Pandemics[MESH] |Pneumonia, Viral[MESH] |Prevalence[MESH] |RNA[MESH] |SARS-CoV-2[MESH] |Spain[MESH] |Swine[MESH]SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low (epmc).pdf DeepDyve Pubget Overpricing